Development and application of molecularly imprinted polymers as solid-phase sorbents for erythromycin extraction
- 469 Downloads
Six molecularly imprinted polymers (MIPs) of erythromycin (ERY) were prepared by noncovalent bulk polymerization using methacrylic acid (MAA) as the functional monomer. On the basis of binding analysis, the MIPs with 1:2 optimum ratio of template to MAA were selected for subsequent scanning electron microscopy and Brunauer–Emmett–Teller analyses, which indicated that the MIPs had more convergent porous structures than the nonimprinted polymers. The equilibrium binding experiments showed that the binding sites of MIPs were heterogeneous, with two dissociation constants of 0.005 and 0.63 mg mL−1, respectively. Furthermore, the performance of the MIPs as solid-phase extraction (SPE) sorbents was evaluated, and the selectivity analysis showed that the MIPs could recognize ERY with moderate cross-reactivity for other macrolides. The overall investigation of molecularly imprinted SPE for cleanup and enrichment of the ERY in pig muscle and tap water confirmed the feasibility of utilizing the MIPs obtained as specific SPE sorbents for ERY extraction in real samples.
KeywordsMolecularly imprinted polymers Erythromycin Solid-phase extraction Pig muscle Tissue sample
The authors acknowledge the financial support from the National “863’’ High-Tech Project (2006AA10Z438), the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China (NO.2006BAK02A09), Shanghai Nano-tech Special Fund (0652nm017) and Shanghai Leading Academic Discipline Project (B205). The authors are grateful to the Instrumental Analysis Center of Shanghai Jiao Tong University, China, for SEM photography. We are grateful to Tom Hsiang (University of Guelph, Canada) for technical language editing.
- 2.European Union (2002) EMEA erythromycin summary report. Committee for Veterinary Medicinal ProductsGoogle Scholar
- 7.Lanza F, Sellergren B (2001) Adv Chromatogr 41:137–173Google Scholar
- 13.Siemann M, Andersson LI, Mosbach K (1997) J Antibiot (Tokyo) 50:89–91Google Scholar
- 16.Yu HJ, Shen XS, Li Q, Huang DM, Chen YN, Guan LD (2006) Chin J Anal Lab 25:82–85Google Scholar
- 28.Andersson LI, Nicholls IA, Mosbach K (1996) Adv Mol Cell Biol 651–670Google Scholar