Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry
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Studies are described on the metabolism and the toxicological analysis of the amphetamine-derived designer drug 4-chloro-2,5-dimethoxyamphetamine (DOC) in rat urine using gas chromatographic-mass spectrometric techniques. The metabolites identified indicated that DOC was metabolized by O-demethylation at position 2 or 5 of the phenyl ring partly followed by glucuronidation and/or sulfation. The authors’ systematic toxicological analysis procedure using full-scan gas chromatography-mass spectrometry after acid hydrolysis, liquid-liquid extraction and microwave-assisted acetylation allowed the detection of an intake of a dose of DOC in rat urine that corresponds to a common drug user’s dose. Assuming similar metabolism, the STA procedure described should be suitable as proof of an intake of DOC in human urine.
Keywords4-Chloro-2,5-dimethoxyamphetamine Designer drug Metabolism Gas chromatography-mass spectrometry Urinalysis
The authors would like to thank Johanna Schäning, Frank T. Peters, Christoph Sauer, Gabi Ulrich and Armin A. Weber for their suggestions and help.
- 1.Shulgin A (1991) Pihkal, a chemical love story. Transform, BerkleyGoogle Scholar
- 6.Drug Enforcement Administration-Office of Forensic Sciences (2006) Microgram 39:137Google Scholar
- 7.Drug Enforcement Administration-Office of Forensic Sciences (2006) Microgram 39:72Google Scholar
- 8.Drug Enforcement Administration-Office of Forensic Sciences (2007) Microgram 40:42Google Scholar
- 9.Drug Enforcement Administration-Office of Forensic Sciences (2007) Microgram 40:93Google Scholar
- 13.McKay AF, Ott WL, Taylor GW, Buchanan MN, Crooker JF (1950) Can J Res 28:683–688Google Scholar
- 15.Maurer HH, Pfleger K, Weber AA (2007) Mass spectral library of drugs, poisons, pesticides, pollutants and their metabolites, 4th edn. Wiley-VCH, WeinheimGoogle Scholar
- 19.McLafferty FW, Turecek F (1993) Interpretation of mass spectra. University Science Books, Mill ValleyGoogle Scholar
- 20.Smith RM, Busch KL (1999) Understanding mass spectra-a basic approach. Wiley, New YorkGoogle Scholar
- 22.Maurer HH, Pfleger K, Weber AA (2007) Mass spectral and GC data of drugs, poisons, pesticides, pollutants and their metabolites, 3rd edn. Wiley-VCH, WeinheimGoogle Scholar
- 24.de-Zeeuw RA, Franke JP, Maurer HH, Pfleger K (1992) Gas chromatographic retention indices of toxicologically relevant substances and their metabolites (report of the DFG commission for clinical toxicological analysis, special issue of the TIAFT bulletin). VCH, WeinheimGoogle Scholar