Analytical and Bioanalytical Chemistry

, Volume 390, Issue 7, pp 1837–1842 | Cite as

Metabolism and toxicological detection of the designer drug 4-chloro-2,5-dimethoxyamphetamine in rat urine using gas chromatography-mass spectrometry

  • Andreas H. Ewald
  • Dorothea Ehlers
  • Hans H. Maurer
Original Paper

Abstract

Studies are described on the metabolism and the toxicological analysis of the amphetamine-derived designer drug 4-chloro-2,5-dimethoxyamphetamine (DOC) in rat urine using gas chromatographic-mass spectrometric techniques. The metabolites identified indicated that DOC was metabolized by O-demethylation at position 2 or 5 of the phenyl ring partly followed by glucuronidation and/or sulfation. The authors’ systematic toxicological analysis procedure using full-scan gas chromatography-mass spectrometry after acid hydrolysis, liquid-liquid extraction and microwave-assisted acetylation allowed the detection of an intake of a dose of DOC in rat urine that corresponds to a common drug user’s dose. Assuming similar metabolism, the STA procedure described should be suitable as proof of an intake of DOC in human urine.

Keywords

4-Chloro-2,5-dimethoxyamphetamine Designer drug Metabolism Gas chromatography-mass spectrometry Urinalysis 

Notes

Acknowledgements

The authors would like to thank Johanna Schäning, Frank T. Peters, Christoph Sauer, Gabi Ulrich and Armin A. Weber for their suggestions and help.

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Andreas H. Ewald
    • 1
  • Dorothea Ehlers
    • 2
  • Hans H. Maurer
    • 1
  1. 1.Department of Experimental and Clinical Toxicology, Institute of Experimental and Clinical Pharmacology and ToxicologySaarland UniversityHomburg (Saar)Germany
  2. 2.cc chemical consultingBerlinGermany

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