Analytical and Bioanalytical Chemistry

, Volume 391, Issue 1, pp 21–31 | Cite as

Comprehensive two-dimensional liquid chromatography coupled with mass spectrometry

  • Jaroslav Pól
  • Tuulia HyötyläinenEmail author


In this review, instrumental aspects of comprehensive two-dimensional liquid chromatography coupled with mass spectrometry are presented. The milestones of LC×LC are briefly summarized. Instrument configuration, selection of experimental conditions, the different interfaces used in the system and the current applications of LC×LC–MS systems are described.


Comprehensive two-dimensional liquid chromatography Mass spectrometry 


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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  1. 1.Department of Chemistry, Laboratory of Analytical ChemistryUniversity of HelsinkiHelsinkiFinland
  2. 2.Division of Pharmaceutical ChemistryUniversity of HelsinkiHelsinkiFinland

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