Analytical and Bioanalytical Chemistry

, Volume 390, Issue 8, pp 2021–2029 | Cite as

Catalytic diesel particulate filters reduce the in vitro estrogenic activity of diesel exhaust

  • Daniela Wenger
  • Andreas C. Gerecke
  • Norbert V. Heeb
  • Hanspeter Naegeli
  • Renato Zenobi
Original Paper

Abstract

An in vitro reporter gene assay based on human breast cancer T47D cells (ER-CALUX®) was applied to examine the ability of diesel exhaust to induce or inhibit estrogen receptor (ER)-mediated gene expression. Exhaust from a heavy-duty diesel engine was either treated by iron- or copper/iron-catalyzed diesel particulate filters (DPFs) or studied as unfiltered exhaust. Collected samples included particle-bound and semivolatile constituents of diesel exhaust. Our findings show that all of the samples contained compounds that were able to induce ER-mediated gene expression as well as compounds that suppressed the activity of the endogenous hormone 17β-estradiol (E2). Estrogenic activity prevailed over antiestrogenic activity. We found an overall ER-mediated activity of 1.63 ± 0.31 ng E2 CALUX equivalents (E2-CEQs) per m3 of unfiltered exhaust. In filtered exhaust, we measured 0.74 ± 0.07 (iron-catalyzed DPF) and 0.55 ± 0.09 ng E2-CEQ m−3 (copper/iron-catalyzed DPF), corresponding to reductions in estrogenic activity of 55 and 66%, respectively. Our study demonstrates that both catalytic DPFs lowered the ER-mediated endocrine-disrupting potential of diesel exhaust.

Keywords

Diesel exhaust Diesel particles Diesel particulate filter In vitro reporter gene assay Estrogen receptor Estrogenic activity 

Abbreviations

AhR

Aryl hydrocarbon receptor

ANOVA

One-way analysis of variance

Cl

Chlorine-enriched diesel fuel (14 μg g−1)

Cu

Diesel fuel with copper/iron-based fuel additive (9.0/7.5 μg g−1)

DMSO

Dimethylsulfoxide

DPF

Diesel particulate filter

E2

17β-Estradiol

EE2

17α-Ethinylestradiol

E2-CEQ

17β-Estradiol CALUX equivalent

ER

Estrogen receptor;

ER-CALUX®

Estrogen Responsive–Chemically Activated LUciferase eXpression

F

Exhaust treatment by diesel particulate filter

FBS

Fetal bovine serum

Fe

Diesel fuel with iron-based additive (4.5 μg g−1)

PCDD/Fs

Polychlorinated dibenzodioxins/furans

Ref

Reference diesel fuel

RSD

Relative standard deviation

xCl

Chlorine-enriched diesel fuel (110 μg g−1)

Notes

Acknowledgements

We thank all our collaborators at Empa, BFH-TI Biel, and TTM Niederrohrdorf for providing exhaust samples and know-how. This study was supported by the board of Empa and the Swiss National Science Foundation (NRP50 program, grant no. 40504–104378).

Supplementary material

216_2008_1872_MOESM1_ESM.doc (548 kb)
ESM 1 (DOC 548 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Daniela Wenger
    • 1
  • Andreas C. Gerecke
    • 1
  • Norbert V. Heeb
    • 1
  • Hanspeter Naegeli
    • 2
  • Renato Zenobi
    • 3
  1. 1.Laboratory for Analytical Chemistry, Empa, Swiss Federal Laboratories for Materials Testing and ResearchDübendorfSwitzerland
  2. 2.Institute of Pharmacology and ToxicologyUniversity of Zurich-VetsuisseZurichSwitzerland
  3. 3.Department of Chemistry and Applied BiosciencesETH ZurichZurichSwitzerland

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