Analytical and Bioanalytical Chemistry

, Volume 391, Issue 3, pp 943–950 | Cite as

Gold nanoparticles for the development of clinical diagnosis methods

  • Pedro Baptista
  • Eulália Pereira
  • Peter Eaton
  • Gonçalo Doria
  • Adelaide Miranda
  • Inês Gomes
  • Pedro Quaresma
  • Ricardo Franco
Review

Abstract

The impact of advances in nanotechnology is particularly relevant in biodiagnostics, where nanoparticle-based assays have been developed for specific detection of bioanalytes of clinical interest. Gold nanoparticles show easily tuned physical properties, including unique optical properties, robustness, and high surface areas, making them ideal candidates for developing biomarker platforms. Modulation of these physicochemical properties can be easily achieved by adequate synthetic strategies and give gold nanoparticles advantages over conventional detection methods currently used in clinical diagnostics. The surface of gold nanoparticles can be tailored by ligand functionalization to selectively bind biomarkers. Thiol-linking of DNA and chemical functionalization of gold nanoparticles for specific protein/antibody binding are the most common approaches. Simple and inexpensive methods based on these bio-nanoprobes were initially applied for detection of specific DNA sequences and are presently being expanded to clinical diagnosis.

Figure

Colorimetric DNA/RNA detection using salt induced aggregation of AuNP-DNA nanoprobes

Keywords

Nanoparticles Gold nanoparticles Nanotechnology Nucleic acids (DNA/RNA) Bioanalytical methods Biological samples 

Abbreviations

DNA

Deoxyribonucleic acid

ssDNA

Single-stranded deoxyribonucleic acid

RNA

Ribonucleic acid

AuNP

Gold nanoparticle

AFM

Atomic force microscopy

SNP

Single-nucleotide polymorphism

SPR

Surface plasmon resonance

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Pedro Baptista
    • 1
  • Eulália Pereira
    • 2
  • Peter Eaton
    • 2
  • Gonçalo Doria
    • 1
    • 3
  • Adelaide Miranda
    • 2
  • Inês Gomes
    • 3
  • Pedro Quaresma
    • 1
    • 2
  • Ricardo Franco
    • 3
  1. 1.CIGMH/Dept. Ciências da VidaFaculdade de Ciências e Tecnologia, Universidade Nova de LisboaCaparicaPortugal
  2. 2.REQUIMTE, Departamento de QuímicaFaculdade de Ciências da Universidade do PortoPortoPortugal
  3. 3.REQUIMTE, Departamento de QuímicaFaculdade de Ciências e Tecnologia, Universidade Nova de LisboaCaparicaPortugal

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