Analytical and Bioanalytical Chemistry

, Volume 390, Issue 4, pp 1087–1091 | Cite as

The study of surface properties of an IgE-sensitive aptasensor using an acoustic method

  • M. Šnejdárková
  • L. Svobodová
  • V. Polohová
  • T. Hianik
Original Paper

Abstract

We applied the acoustic transverse shear mode (TSM) method for study of the surface properties of a DNA aptasensor that specifically binds human immunoglobulin E (IgE). The biotinylated 45-mer DNA aptamers were immobilized on the surface of a self-assembled layer composed of a mixture of polyamidoamine dendrimers of the fourth generation with 1-hexadecanetiol covered by neutravidin. Using the TSM method, we studied the kinetics of changes of the series resonant frequency, fs, and the motional resistance, Rm, of a quartz crystal transducer, used as a support for formation of the sensing layer. We have shown that attachment of the biotinylated DNA aptamers onto the surface covered by neutravidin results in a decrease of fs, but in an increase of Rm. Similar changes of fs and Rm were observed following addition of IgE. This suggests the contribution of friction forces to the crystal oscillation, which was taken into account in the calculation of the mass changes at the sensor surface following binding processes.

Keywords

DNA aptamer Immunoglobulin E Dendrimers Transverse shear mode method Biosensor 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • M. Šnejdárková
    • 1
  • L. Svobodová
    • 1
  • V. Polohová
    • 1
  • T. Hianik
    • 2
  1. 1.Institute of Animal Biochemistry and GeneticsSlovak Academy of SciencesIvanka pri DunajiSlovak Republic
  2. 2.Department of Nuclear Physics and BiophysicsComenius UniversityBratislavaSlovak Republic

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