Analytical and Bioanalytical Chemistry

, Volume 389, Issue 5, pp 1469–1475 | Cite as

Metabolic profiling using Fourier-transform ion-cyclotron-resonance mass spectrometry

Technical Note

Abstract

With the aid of the extreme resolving power of Fourier-transform ion-cyclotron-resonance mass spectrometry (FT-ICR/MS), we have developed a metabolomics platform for high-throughput metabolic profiling and metabolite candidate identification integrating a data-processing system, the Dr.DMASS program (http://kanaya.naist.jp/DrDMASS/), and a metabolite-species database, KNApSAcK (http://kanaya.aist-nara.ac.jp/KNApSAcK/). We discuss the potential of this FT-ICR/MS-based metabolic profiling scheme as a general metabolomics tool by clarification of plant metabolic disorders and specific metabolite accumulation patterns caused by herbicidal enzyme inhibitors.

Keywords

Fourier-transform ion-cyclotron-resonance mass spectrometry Metabolomics Data processing 

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Daisaku Ohta
    • 1
  • Daisuke Shibata
    • 3
  • Shigehiko Kanaya
    • 2
  1. 1.Graduate School of Life and Environmental SciencesOsaka Prefecture UniversitySakaiJapan
  2. 2.Graduate School of Information ScienceNara Institute of Science and TechnologyIkomaJapan
  3. 3.Kazusa DNA Research InstituteKisarazuJapan

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