Analytical and Bioanalytical Chemistry

, Volume 389, Issue 4, pp 1177–1183 | Cite as

In situ synthesis of molecularly imprinted polymers on glass microspheres in a column

  • Yan Zhuang
  • Hongpeng Luo
  • Deliang Duan
  • Lirong Chen
  • Xiaojie XuEmail author
Short Communication


A facile method to fabricate molecularly imprinted polymers (MIPs) on glass microspheres in a column was developed. The column was prepacked with glass microspheres, and then the prepolymerization mixture was injected into the interstitial volume of the column. The polymerization took place in situ and the column could be directly used for high-performance liquid chromatography after the template had been removed. The template consumption was reduced greatly because the prepolymerization mixture just filled the interstitial volume between the glass microspheres in the column. The MIPs obtained exhibited better kinetic properties, higher efficiency, and low back pressure of the column. Emodin imprinted polymers were prepared by this method and were used for solid-phase extraction.


Molecularly imprinted polymer In-column Glass microsphere Emodin Solid-phase extraction 



We would like to thank the National Natural Science Foundation of China for financial support (NSFC20375002).


  1. 1.
    Vlatakis G, Anderson LI, Müller R, Mosbach K (1993) Nature 361:645–647CrossRefGoogle Scholar
  2. 2.
    Wulff G (1995) Angew Chem Int Ed Engl 34:1812–1832CrossRefGoogle Scholar
  3. 3.
    Whitcombe MJ, Rodriguez ME, Villar P, Vulfson E (1995) J Am Chem Soc 117:7105–7111CrossRefGoogle Scholar
  4. 4.
    Haupt K, Mosbach K (1998) Trends Biotechnol 16:468–475CrossRefGoogle Scholar
  5. 5.
    Haupt K (2003) Anal Chem A 75:376A–383AGoogle Scholar
  6. 6.
    Klein JU, Whitcombe MJ, Mulholland F, Vulfson EN (1999) Angew Chem Int Ed Engl 38:2057–2060CrossRefGoogle Scholar
  7. 7.
    Pérez-Moral N, Mayes AG (2004) Anal Chim Acta 504:15–21CrossRefGoogle Scholar
  8. 8.
    Mayes AG, Mosbach K (1996) Anal Chem 68:3769–3774CrossRefGoogle Scholar
  9. 9.
    Strikovsky AG, Kasper D, Grun M, Green BS, Hradil J, Wulff GJ (2000) J Am Chem Soc 122:6295–6296CrossRefGoogle Scholar
  10. 10.
    Zourob M, Mohr S, Mayes AG, Macaskill A, Pérez-Moral N, Fieldena PR, Goddard NJ (2006) Lab Chip 6:296–301CrossRefGoogle Scholar
  11. 11.
    Ye L, Cormack AG, Mosbach K (1999) Anal Commun 36:35–38CrossRefGoogle Scholar
  12. 12.
    Ye L, Weiss R, Mosbach K (2000) Macromolecules 33:8239–8245CrossRefGoogle Scholar
  13. 13.
    Boonpangrak S, Prachayasittikul V, Bülow L, Ye L (2006) J Appl Polym Sci 99:1390–1398CrossRefGoogle Scholar
  14. 14.
    Hosoya K, Fréchet JM (1993) J Polym Sci Polym Chem 21:2129–2141CrossRefGoogle Scholar
  15. 15.
    Pérez N, Whitcombe MJ, Vulfson EN (2000) J Appl Polym Sci 77:1851–1859CrossRefGoogle Scholar
  16. 16.
    Pérez N, Whitcombe MJ, Vulfson EN (2001) Macromolecules 34:830–836CrossRefGoogle Scholar
  17. 17.
    Sulitzky C, Rückert B, Hall AJ, Lanza F, Unger K, Sellergren B (2002) Macromolecules 35:79–91CrossRefGoogle Scholar
  18. 18.
    Sellergren B, Rückert B, Hall AJ (2002) Adv Mater 14:1204–1208CrossRefGoogle Scholar
  19. 19.
    Tamayo FG, Titirici MM, Martin-Esteban A, Sellergren B (2005) Anal Chim Acta 542:38–46CrossRefGoogle Scholar
  20. 20.
    Piletsky SA, Matuschewski H, Schedler U, Wilpert A, Piletska EV, Thiele TA, Ulbricht M (2000) Macromolecules 33:3092–3098CrossRefGoogle Scholar
  21. 21.
    Rückert B, Hall AJ, Sellergren B (2002) J Mater Chem 12:2275–2280CrossRefGoogle Scholar
  22. 22.
    Courtois J, Fischer G, Sellergren B, Irguma K (2006) J Chromatogr A 1109:92–99CrossRefGoogle Scholar
  23. 23.
    Matsui J, Nicholls IA, Takeuchi T (1998) Anal Chim Acta 365:89–93CrossRefGoogle Scholar
  24. 24.
    Li H, Liu Y, Zhang Z, Liao H, Nie L, Yao S (2005) J Chromatogr A 1098:66–74CrossRefGoogle Scholar
  25. 25.
    Kuo YC, Meng HC, Tsai WJ (2001) Inflamm Res 50:73–82CrossRefGoogle Scholar
  26. 26.
    Lee HZ (2001) Br J Pharmacol 134:11–20CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Yan Zhuang
    • 1
  • Hongpeng Luo
    • 1
  • Deliang Duan
    • 1
  • Lirong Chen
    • 1
  • Xiaojie Xu
    • 1
    Email author
  1. 1.College of Chemistry and Molecular EngineeringPeking UniversityBeijingChina

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