Analytical and Bioanalytical Chemistry

, Volume 389, Issue 4, pp 1193–1199

Detection of avian influenza virus using an interferometric biosensor

Original Paper

DOI: 10.1007/s00216-007-1525-3

Cite this article as:
Xu, J., Suarez, D. & Gottfried, D.S. Anal Bioanal Chem (2007) 389: 1193. doi:10.1007/s00216-007-1525-3

Abstract

An interferometric biosensor immunoassay for direct and label-less detection of avian influenza through whole virus capture on a planar optical waveguide is described. The assay response is based on index of refraction changes that occur upon binding of virus particles to unique antigen-specific (hemagglutinin) antibodies on the waveguide surface. Three virus subtypes (two H7 and one H8) in buffer solution were tested using both monoclonal and polyclonal capture antibodies. The real-time response of the antigen–antibody interaction was measured and was shown to be concentration-dependent, with detection limits as low as 0.0005 hemagglutination units per milliliter. A simple sandwich assay was shown to further increase the biosensor response.

Figure

Response of the optical interferometric biosensor (inset) to avian influenza virus using a monoclonal antibody (mAb) for whole virus capture in a direct binding assay. The response of the anti-H7 mAb is specific to the H7 subtype

Keywords

Optical waveguide Interferometer Immunoassay Antibody Hemagglutinin 

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Georgia Tech Research InstituteGeorgia Institute of TechnologyAtlantaUSA
  2. 2.Southeast Poultry Research LabAgricultural Research Service, USDAAthensUSA
  3. 3.Microelectronics Research CenterGeorgia Institute of TechnologyAtlantaUSA

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