Analytical and Bioanalytical Chemistry

, Volume 390, Issue 3, pp 817–823 | Cite as

Micro- and nanometer-scale patterned surface in a microchannel for cell culture in microfluidic devices

  • Makiko Goto
  • Takehiko Tsukahara
  • Kiichi Sato
  • Takehiko Kitamori
Original Paper


A novel microdevice which had a micro- and nanometer-scale patterned surface for cell adhesion in a microchip was developed. The surface had a metal pattern fabricated by electron-beam lithography and metal sputtering and a chemical pattern consisting of a self-assembled monolayer of alkanethiol. The metal patterned surface had a gold stripe pattern which was as small as 300 nm wide and 150 nm high and both topography and chemical properties could be controlled. Mouse fibroblast NIH/3T3 cells were cultured on the patterned surface and elongated along the gold stripes. These cells recognized the size of the pattern and the chemical properties on the pattern though it was much smaller than they were. There was satisfactory cell growth under fresh medium flow in the microchip. The combination of the patterned surface and the microchip provides cells with a novel environment for their growth and will facilitate many cellular experiments.


Micro- and nanometer-scale patterned surface Nanofabrication Self-assembled monolayer Cell adhesion Cell elongation 

Supplementary material

216_2007_1496_MOESM1_ESM.pdf (567 kb)
ESM 1(PDF 566 kb)


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Makiko Goto
    • 1
    • 2
  • Takehiko Tsukahara
    • 1
    • 2
  • Kiichi Sato
    • 3
  • Takehiko Kitamori
    • 1
    • 2
  1. 1.Department of Applied Chemistry, School of EngineeringThe University of TokyoTokyoJapan
  2. 2.Core Research for Evolutional Science and TechnologyJapan Science and Technology AgencyTokyoJapan
  3. 3.Department of Applied Biological ChemistrySchool of Agriculture and Life Sciences, The University of TokyoTokyoJapan

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