Analytical and Bioanalytical Chemistry

, Volume 389, Issue 2, pp 455–460 | Cite as

Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking

  • Claire Rossi
  • Karsten HauptEmail author
Technical Note


We propose the use of Doehlert’s experimental design, a second-order uniform shell design, for the optimization of molecularly imprinted polymers (MIPs). We have chosen a simple model system where the influence of kind and degree of cross-linking on template recognition was studied using S-propranolol as the template. We found that Doehlert’s design allows—with very few experiments—one to screen the evolution of the binding capacity of a MIP as a function the different parameters, and thus appears to be a powerful means to screen for the best composition and synthesis method for MIPs. We believe that this chemometric tool can significantly accelerate the development of new MIPs as synthetic recognition elements, particularly in the context of a given application, and will be a versatile complement or alternative to first-order designs to fit complex processes.


Chemometrics Statistics Molecularly imprinted polymers (MIP) Polymers 

Supplementary material

216_2007_1484_MOESM1_ESM.pdf (63 kb)
ESM 1 Application of the Doehlert experimental design to molecularly imprinted polymers: surface response optimization of specific template recognition as a function of the type and degree of cross-linking. (PDF 64 kb)


  1. 1.
    Arshady R, Mosbach K (1981) Makromol Chem 182:687–692CrossRefGoogle Scholar
  2. 2.
    Wulff G, Sarhan A (1972) Angew Chem Int Ed 11:341Google Scholar
  3. 3.
    Sellergren B (2001) Molecularly imprinted polymers - man-made mimics of antibodies and their applications in analytical chemistry. Elsevier, AmsterdamGoogle Scholar
  4. 4.
    Chianella I, Lotierzo M, Piletsky SA, Tothill IE, Chen B, Karim K, Turner APF (2002) Anal Chem 74:1288–1293CrossRefGoogle Scholar
  5. 5.
    Takeuchi T, Fukuma D, Matsui J (1999) Anal Chem 71:285–290CrossRefGoogle Scholar
  6. 6.
    Lanza F, Sellergren B (1999) Anal Chem 71:2092–2096CrossRefGoogle Scholar
  7. 7.
    Dirion B, Cobb Z, Schillinger E, Andersson LI, Sellergren B (2003) J Am Chem Soc 125:15101–15109CrossRefGoogle Scholar
  8. 8.
    Baggiani C, Anfossi L, Giovannoli C, Tozzi C (2004) J Chromatogr B 804:31–41CrossRefGoogle Scholar
  9. 9.
    Navarro-Villoslada F, San Vicente B, Moreno-Bondi MC (2004) Anal Chim Acta 504:149–162CrossRefGoogle Scholar
  10. 10.
    Rosengren AM, Karlsson JG, Andersson PO, Nicholls IA (2005) Anal Chem 77:5700–5705CrossRefGoogle Scholar
  11. 11.
    Kempe H, Kempe M (2004) Macromol Rapid Comm 25:315–320CrossRefGoogle Scholar
  12. 12.
    Navarro-Villoslada F, Takeuchi T (2005) Bull Chem Soc Jpn 78:1354–1361CrossRefGoogle Scholar
  13. 13.
    Doehlert DH (1970) Appl Stats 19:231–239CrossRefGoogle Scholar
  14. 14.
    Ferreira S (2004) Talanta 63:1061–1067CrossRefGoogle Scholar
  15. 15.
    Gonzalez G, Gonzalez-Arjona D (1999) Talanta 49:433–439CrossRefGoogle Scholar
  16. 16.
    Hounsa CG, Aubry JM, Dubourguier HC, Hornez JP (1996) Appl Microbiol Biotechnol 45:764–770CrossRefGoogle Scholar
  17. 17.
    Furlanetto S, Pinzauti S, La Porta E, Chiarugi A, Mura P, Orlandini S (1998) J Pharm Biomed Anal 17:1015–1028CrossRefGoogle Scholar
  18. 18.
    Garrido-Vidal D, Pizzaro C, Gonzales-Saiz J (2003) Biotechnol Prog 19:1468–1479CrossRefGoogle Scholar
  19. 19.
    dos Santos WL, dos Santos CM, Costa JL, Andrade HM, Ferreira S (2004) Microchem J 77:123–129CrossRefGoogle Scholar
  20. 20.
    Linder M, Kochanovski N, Fanni J, Parmentier M (2005) Process Biochem 40:273–279CrossRefGoogle Scholar
  21. 21.
    Andersson LI (1996) Anal Chem 68:111–117CrossRefGoogle Scholar
  22. 22.
    Kempe M (1996) Anal Chem 68:1948–1953CrossRefGoogle Scholar
  23. 23.
    Mayes AG, Mosbach K (1996) Anal Chem 68:3769–3774CrossRefGoogle Scholar
  24. 24.
    Yu C, Mosbach K (2000) J Chromatogr A 888:63–72CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.UMR CNRS 6022 - Centre de Recherches de RoyallieuCompiègne University of TechnologyCompiègne cedexFrance

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