Analytical and Bioanalytical Chemistry

, Volume 389, Issue 6, pp 1773–1789 | Cite as

Analysis of pesticide residues in fruit and vegetables with ethyl acetate extraction using gas and liquid chromatography with tandem mass spectrometric detection

  • Tuija Pihlström
  • Gun Blomkvist
  • Paula Friman
  • Ulla Pagard
  • Bengt-Göran Österdahl
Original Paper


A multiresidue method based on extraction with ethyl acetate has been used at the Swedish National Food Administration since 1989 to monitor pesticide residues in fruit and vegetables. The method has been continuously adjusted, resulting in simple and quick analyses of pesticide residues. To recover basic pesticides, the addition of an alkali is necessary. The addition of sodium hydrogen carbonate has been shown to recover all pesticides effectively without any degradation. The liquid chromatography (LC) with tandem mass spectrometry (MS/MS) technique has made it possible to analyse more polar pesticides and to replace many single methods. The latest development in the multiresidue method, comprising the use of gas chromatography (GC) with MS/MS, has further improved the analysis by replacing the conventional GC detectors. The need for cleanup has been reduced or eliminated entirely. Consequently, the method has been simplified in a way that makes it possible to recover all included analytes in many different matrices in one single extraction and to detect them either with GC-MS/MS or with LC-MS/MS.


Pesticides Multiresidue analysis GC-MS/MS and LC-MS/MS Fruit and vegetables 



We gratefully acknowledge the co-operation and the diligent and skilful experimental work of Kristina Nordmark, Cathrin Landegren, Aida Pivodic and Jesus Regalado at Analycen Lantmännen, Lidköping, Sweden, and Anna Liedgren at Chemistry Division 1, NFA, Uppsala, Sweden.


  1. 1.
    Luke MA, Froberg JE, Masumo HT (1975) J Assoc Off Anal Chem Int 58:1020Google Scholar
  2. 2.
    Andersson A, Pålsheden H (1991) Fresenius J Anal Chem 339:365CrossRefGoogle Scholar
  3. 3.
    Andersson A, Pålsheden H, Arén B (1996) Poster presentation at the 1st European pesticide residue workshop, Alkmaar, The NetherlandsGoogle Scholar
  4. 4.
    Valverde A, Pihlström T, Andersson A (1997) Anal Chim Acta 338:63–68CrossRefGoogle Scholar
  5. 5.
    Taylor MJ, Hunter KB, Lindsay D, Le Bouhellec S (2002) J Chromatogr A 982:225CrossRefGoogle Scholar
  6. 6.
    Barnes K, Fussell RJ, Startin J, Pegg MK, Thorpe SA, Reynolds SL (1997) Mass Spectrom 11:117Google Scholar
  7. 7.
    Barnes K, Fussell RJ, Startin J, Pegg MK, Thorpe SA, Reynolds SL (1997) Mass Spectro 11:159Google Scholar
  8. 8.
    Anastassiades M, Lehotay SJ, Šteinbaher D, Schenk FJ (2003) J AOAC Int 86:412–431Google Scholar
  9. 9.
    Gamon M, Leo C, Ten A, Mocholi F (2001) J AOAC Int 84: 1209–1216Google Scholar
  10. 10.
    Leandro CC, Hancock P, Fussell R, Keely BJ (2006) J Chromatogr A 1103:94–101CrossRefGoogle Scholar
  11. 11.
    Ferrer I, Garcia-Reyes JF, Mezua M, Thurman EM, Fernandez-Alba AR (2005) J Chromatogr A 108:81–90CrossRefGoogle Scholar
  12. 12.
    Alder L, Greulich K, Kempe G, Vieh B (2006) Mass Spectrom 6:838–865CrossRefGoogle Scholar
  13. 13.
    Hernandez F, Pozo OJ, Sancho JV, Bijlsma L, Barreda A, Pitarch E (2006) J Chromatogr A 1109:242–252CrossRefGoogle Scholar
  14. 14.
    Jansson C, Pihlström T, Österdahl BG, Markides K (2004) J Chromatogr A 1023:93–104CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Tuija Pihlström
    • 1
  • Gun Blomkvist
    • 1
  • Paula Friman
    • 2
  • Ulla Pagard
    • 1
  • Bengt-Göran Österdahl
    • 1
  1. 1.Research and Development DepartmentNational Food AdministrationUppsalaSweden
  2. 2.Lantmännen Analycen ABLidköpingSweden

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