Analytical and Bioanalytical Chemistry

, Volume 389, Issue 1, pp 93–109 | Cite as

Analysis of bovine immunoglobulin G in milk, colostrum and dietary supplements: a review

  • Leyton W. Gapper
  • David E. J. Copestake
  • Don E. Otter
  • Harvey E. Indyk


The immunoprotective properties of bovine milk immunoglobulin G (IgG) have led to a recent proliferation of nutritional products incorporating this protein. It has therefore become critical that reliable analytical techniques for the measurement of the IgG content in such products are available. This literature review surveys current methods of analysis for IgG, including separation-based or immuno-based concentration analysis. The review also discusses nutraceutical applications, regulatory issues, stability of IgG and the significance of primary reference material in IgG analysis.


Immunoglobulin G IgG Bovine Milk Colostrum Protein Analysis Dietary supplements 



Affinity chromatography


Capillary electrophoresis


Capillary gel electrophoresis


Capillary zone electrophoresis


Enzyme-linked immunosorbent assay




Ion exchange chromatography




Immunoglobulin G


Liquid chromatography


Radial immunodiffusion


Reversed-phase liquid chromatography


Sodium dodecyl sulphate polyacrylamide gel electrophoresis


Size exclusion chromatography


Surface plasmon resonance


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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Leyton W. Gapper
    • 1
  • David E. J. Copestake
    • 1
  • Don E. Otter
    • 1
  • Harvey E. Indyk
    • 2
  1. 1.Fonterra Research CentrePalmerston NorthNew Zealand
  2. 2.Fonterra WaitoaWaitoaNew Zealand

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