Analytical and Bioanalytical Chemistry

, Volume 389, Issue 1, pp 93–109 | Cite as

Analysis of bovine immunoglobulin G in milk, colostrum and dietary supplements: a review

  • Leyton W. Gapper
  • David E. J. Copestake
  • Don E. Otter
  • Harvey E. Indyk
Review

Abstract

The immunoprotective properties of bovine milk immunoglobulin G (IgG) have led to a recent proliferation of nutritional products incorporating this protein. It has therefore become critical that reliable analytical techniques for the measurement of the IgG content in such products are available. This literature review surveys current methods of analysis for IgG, including separation-based or immuno-based concentration analysis. The review also discusses nutraceutical applications, regulatory issues, stability of IgG and the significance of primary reference material in IgG analysis.

Keywords

Immunoglobulin G IgG Bovine Milk Colostrum Protein Analysis Dietary supplements 

Abbreviations

AC

Affinity chromatography

CE

Capillary electrophoresis

CGE

Capillary gel electrophoresis

CZE

Capillary zone electrophoresis

ELISA

Enzyme-linked immunosorbent assay

IA

Immunoassay

IEC

Ion exchange chromatography

Ig

Immunoglobulin

IgG

Immunoglobulin G

LC

Liquid chromatography

RID

Radial immunodiffusion

RP-LC

Reversed-phase liquid chromatography

SDS-PAGE

Sodium dodecyl sulphate polyacrylamide gel electrophoresis

SEC

Size exclusion chromatography

SPR

Surface plasmon resonance

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Leyton W. Gapper
    • 1
  • David E. J. Copestake
    • 1
  • Don E. Otter
    • 1
  • Harvey E. Indyk
    • 2
  1. 1.Fonterra Research CentrePalmerston NorthNew Zealand
  2. 2.Fonterra WaitoaWaitoaNew Zealand

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