Analytical and Bioanalytical Chemistry

, Volume 389, Issue 1, pp 251–261 | Cite as

Chromatographic fingerprint analysis for evaluation of Ginkgo biloba products

  • Pei ChenEmail author
  • Mustafa Ozcan
  • James Harnly
Original Paper


The flavonoids and the terpene lactones are regarded as the two main active components of Ginkgo biloba that affect human health. In the work discussed in this paper, two analytical methods for the characterization of G. biloba authentic materials and commercial products, an LC–UV chromatographic fingerprinting method and a traditional flavonol quantification method, were compared. The traditional method was used to determine the total flavonol content (as glycosides) after acid hydrolysis. The fingerprinting method examined the chromatographic profiles of methanol–water extracts using chemometric methods. The traditional method showed that all the commercial products met the current voluntary standard of 24% flavonols by weight. The chromatographic fingerprinting method revealed significant variations in the commercial products with regard to the relative concentration of individual flavonols.


Ginkgo biloba HPLC UV Dietary supplement Fingerprint 



This research was supported by the Office of Dietary Supplements at the National Institutes of Health under an Interagency Agreement. We thank Dr Lane C. Sander of NIST for providing the NIST Ginkgo Standard Reference Materials.


  1. 1.
    Michel PF (1986) Presse Med 15:1450–1454Google Scholar
  2. 2.
    Gaby AR (1996) Alt Med Rev 1:236–242Google Scholar
  3. 3.
    Strømgaard K, Vogensen SB, Nakanishi K (2005) Encyclopedia of dietary supplements. Marcel Dekker, New York, pp. 249–257Google Scholar
  4. 4.
    Dubber MJ, Kanfer I (2004) J Pharm Sci 7:3030–3039Google Scholar
  5. 5.
    Wu Y, Wu Z, Butko P, Christen Y, Lambert MP, Klein WL, Link CD, Luo Y (2006) J Neurosci 26:13102–13113CrossRefGoogle Scholar
  6. 6.
    Yamamoto Y, Adachi Y, Fujii Y, Kamei C (2007) Brain Res 1128:70–78CrossRefGoogle Scholar
  7. 7.
    Mdzinarishvili A, Kiewert C, Kumar V, Hillert M, Klein J (2007) Neuroscience 144:217–222CrossRefGoogle Scholar
  8. 8.
    van Beek TA (2002) J Chromatogr A 967:21–55CrossRefGoogle Scholar
  9. 9.
    Watson DG, Oliveria EJ (1999) J Chromatogr B 723:203–210CrossRefGoogle Scholar
  10. 10.
    Hasler A, Sticher O (1992) J Chromatogr 605:41–48CrossRefGoogle Scholar
  11. 11.
    Herbal Medicine Expanded Commission E Monographs (2000) American Botanical Council, 6200 Manor Rd, Austin, TXGoogle Scholar
  12. 12.
    DeFeudis FV (1998) G. biloba extract (EGb 761): from chemistry to the clinic. Ullstein Medical, Wiesbaden, GermanyGoogle Scholar
  13. 13.
    Ding C, Chen E, Zhou W, Lindsay RC (2004) Anal Chem 76:4332–4336CrossRefGoogle Scholar
  14. 14.
    Ding C, Chen E, Lindsay RC (2005) Anal Chem 77:2966–2970CrossRefGoogle Scholar
  15. 15.
    Jensen AG, Ndjoko K, Wolfender JL, Hostettmann K, Camponovo F, Soldati F (2002) Phytochem Anal 13:31–38CrossRefGoogle Scholar
  16. 16.
    Li W, Fitzloff JF (2002) J Pharm Biomed Anal 30:67–75CrossRefGoogle Scholar
  17. 17.
    Mauri P, Migliazza B, Pietta P (1999) J Mass Spectrom 34:1361–1367CrossRefGoogle Scholar
  18. 18.
    AHPA (2006) Code of ethics and business conduct. American Herbal Products AssociationGoogle Scholar
  19. 19.
    Ozcan M, Mcauley B, Chen P (2007) J Food Drug Anal 15:55–62Google Scholar
  20. 20.
    Wagner H, Bladt S, Hartmann U, Daily A, Berkulin W (1989) Deutisch Apothek Zeit 129:2421–2424Google Scholar
  21. 21.
    Li W, Fitzloff JF (2002) J Liq Chromatogr Related Technol 25:2501–2514CrossRefGoogle Scholar
  22. 22.
    Sticher O, Meier B, Hasler A (2000) The analysis of G. biloba flavonoids. In: van Beek TA (ed) G. biloba. Harwood, Amsterdam, pp 179–202Google Scholar
  23. 23.
    Gong F, Liang YZ, Xie PS, Chau FT (2003) J Chromatogr A 1002:25–40CrossRefGoogle Scholar
  24. 24.
    Gong F, Liang YZ, Fung YS, Chau FT (2004) J Chromatogr A 1029:173–183CrossRefGoogle Scholar
  25. 25.
    Zeng ZD, Liang YZ, Wang YL, Li XR, Laing LM, Xu QS, Zhao CX, Li BY, Chau FT (2006) J Chromatogr A 1107:273–285CrossRefGoogle Scholar
  26. 26.
    Zeng ZD, Liang YZ, Chau FT, Wang YL (2006) Anal Bioanal Chem 1107:392–400CrossRefGoogle Scholar
  27. 27.
    van Nederkassel AM, Vijverman V, Massart DL, Vander Heyden Y (2005) J Chromatogr A 1085:230–239CrossRefGoogle Scholar
  28. 28.
    Ji YB, Xu QS, Hu YZ, Heyden YV (2005) J Chromatogr A 1066:97–104CrossRefGoogle Scholar
  29. 29.
    Ji YB, Aleart G, Xu CJ, Hu YZ, Heyden YV (2006) J Chromatogr A 1128:273–281CrossRefGoogle Scholar
  30. 30.
    Xie P, Chen S, Liang YZ, Wang X, Tian R, Upton R (2006) J Chromatogr A 1112:171–180CrossRefGoogle Scholar
  31. 31.
    Liang YZ, Xie P, Chan K (2004) J Chromatogr B 812:53–70CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  1. 1.Food Composition LaboratoryUS Department of Agriculture, Agricultural Research Service, Beltsville Human Nutrition Research CenterBeltsvilleUSA

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