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Analytical and Bioanalytical Chemistry

, Volume 389, Issue 2, pp 405–412 | Cite as

Molecularly imprinted microspheres and nanospheres for di(2-ethylhexyl)phthalate prepared by precipitation polymerization

  • Jia-Ping Lai
  • Min-Li Yang
  • Reinhard Niessner
  • Dietmar KnoppEmail author
Original Paper

Abstract

Molecularly imprinted microspheres (MIMs, >3 μm) and nanospheres (MINs, ≈450 nm) for the environmental endocrine disruptor di(2-ethylhexyl)phthalate (DEHP) were prepared by a precipitation polymerization (PP) procedure. The effect of the dispersive solvents acetonitrile (ACN) and cyclohexane (CH), the cross-linkers ethylene glycol dimethacrylate (EDMA) and trimethylpropane trimethacrylate (TRIM), and the template on particle size and morphology of polymers was investigated in detail by scanning electron microscopy (SEM) and BET adsorption isotherm determination. When used as HPLC stationary phase, the microspheres exhibited strong affinity for the template DEHP with an imprint factor (IF) higher than 8.0 in ACN/water (60:40, v/v) as mobile phase. Furthermore, baseline separation of DEHP from benzyl butyl phthalate (BBP) and dibutyl phthalate (DBP) could be achieved. In contrast, no or only poor separation could be observed with non-imprinted polymeric polymers (NIPs) or imprinted bulk polymers (MIB), respectively. Similarly, the obtained MINs exhibited an imprinting effect in pure ACN, i.e. the bond amount of DEHP was significantly higher compared to NIPs, as was shown in rebinding experiments. Besides their use as an HPLC stationary phase, MIMs might further be applicable for SPE sample cleanup, while MINs could be used as a recognition layer on sensor surfaces.

Figure

Molecularly imprinting of di(2-ethylhexyl)phthalate (DEHP)

Keywords

Molecular imprinting Precipitation polymerization Microspheres Nanospheres Di(2-ethylhexyl)phthalate HPLC 

Notes

Acknowledgements

We gratefully acknowledge the financial support by the European Commission (contract number QLK4-CT2002-02323). Further, we thank Dr. Rolf Nitzsche (Malvern Instruments GmbH, Herrenberg, Germany) for performing dynamic light scattering measurements with the Zetasizer Nano ZS instrument. Xaver Hecht (Department of Chemistry, Technische Universität München) is thanked for performing physisorption measurements.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jia-Ping Lai
    • 1
    • 2
  • Min-Li Yang
    • 1
    • 3
  • Reinhard Niessner
    • 1
  • Dietmar Knopp
    • 1
    Email author
  1. 1.Chair of Analytical ChemistryTechnische Universität MünchenMünchenGermany
  2. 2.College of Chemistry & MaterialsSichuan Normal UniversityChengduChina
  3. 3.Chinese Academy of Inspection and Quarantine, Institute of Food SafetyBeijingChina

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