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Analytical and Bioanalytical Chemistry

, Volume 388, Issue 4, pp 881–887 | Cite as

Comparison of GC–MS and LC–MS methods for the analysis of antioxidant phenolic acids in herbs

  • Maarit Kivilompolo
  • Vít Obůrka
  • Tuulia Hyötyläinen
Paper in Forefront

Abstract

Two methods were developed for the quantitative analysis of phenolic acids in herb extracts. The methods were based on liquid chromatography–time-of-flight mass spectrometry (LC–TOFMS) and gas chromatography–mass spectrometry (GC–MS). The methods were compared in terms of their linearity, repeatability, selectivity, sensitivity and the speed of the analysis. The sensitivity was good for both methods, with limits of detection of <80 ng/ml for most of the compounds. The relative standard deviations (RSD) of the peak areas were on average 7.2% for the LC–TOFMS method and 1.4% for the GC–MS method. Both methods were found to be suitable for the determination of the target analytes, although GC–MS was better suited to the quantitative determination of compounds present at low concentrations.

Keywords

Liquid chromatography Gas chromatography Lamiaceae Phenolic acids Mass spectrometry 

Notes

Acknowledgements

We thank Matti Jussila for technical assistant. Funding from the Academy of Finland (Project No. 110429) is gratefully acknowledged.

References

  1. 1.
    Bozin B, Mimica-Dukic N, Simin N, Anackov G (2006) J Agric Food Chem 54:1822–1828Google Scholar
  2. 2.
    Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M (2005) Food Chem 90:333–340Google Scholar
  3. 3.
    Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S, Radice M, Bruni R (2005) Food Chem 91:621–632Google Scholar
  4. 4.
    Ho C-H, Wang M, Wei G-J, Huang T-C, Huang M-T (2000) Biofactors 13:161–166Google Scholar
  5. 5.
    Koşar M, Dorman HJD, Can Başer KH, Hiltunen R (2004) J Agric Food Chem 52:5004–5010PubMedCrossRefGoogle Scholar
  6. 6.
    Shan B, Cai YZ, Sun M, Corke H (2005) J Agric Food Chem 53:7749–7759PubMedCrossRefGoogle Scholar
  7. 7.
    Zheng W, Wang SY (2001) J Agric Food Chem 49:5165–5170PubMedCrossRefGoogle Scholar
  8. 8.
    Areias FM, Valentão P, Andrade PB, Ferreres F, Seabra RM (2001) Food Chem 73:307–311CrossRefGoogle Scholar
  9. 9.
    Ibañez E, Cifuentes A, Crego AL, Senõráns FJ, Cavero S, Reglero G (2000) J Agric Food Chem 48:4060–4065PubMedCrossRefGoogle Scholar
  10. 10.
    Proestos C, Boziaris IS, Nychas G-JE, Komaitis M (2006) Food Chem 95:664–671CrossRefGoogle Scholar
  11. 11.
    Zgórka G, Głowniak K (2001) J Pharm Biomed Anal 26:79–87PubMedCrossRefGoogle Scholar
  12. 12.
    Daferera DJ, Ziogas BN, Polissiou MG (2000) J Agric Food Chem 48:2576–2581PubMedCrossRefGoogle Scholar
  13. 13.
    Venskutonis PR (1997) Food Chem 59:219–227CrossRefGoogle Scholar
  14. 14.
    Minuti L, Pellegrino RM, Tesei I (2006) J Chromatogr A 1114:263–268PubMedCrossRefGoogle Scholar
  15. 15.
    Proestos C, Sereli D, Komaitis M (2006) Food Chem 95:44–52CrossRefGoogle Scholar
  16. 16.
    Ollanketo M, Peltoketo A, Hartonen K, Hiltunen R, Riekkola M-L (2002) Eur Food Res Technol 215:158–163CrossRefGoogle Scholar
  17. 17.
    Ibañez E, Kubátová A, Señoráns FJ, Cavero S, Reglero G, Hawthorne SB (2003) J Agric Food Chem 51:375–382PubMedCrossRefGoogle Scholar
  18. 18.
    Cavero S, Jaime L, Martín-Álvarez PJ, Señoráns FJ, Reglero G, Ibañez E (2005) Eur Food Res Technol 221:478–486CrossRefGoogle Scholar
  19. 19.
    Bicchi C, Binello A, Rubiolo P (2000) Phytochem Anal 11:236–242CrossRefGoogle Scholar
  20. 20.
    Ibañez E, Oca A, de Murga G, López-Sebastián S, Tabera J, Reglero G (1999) J Agric Food Chem 47:1400–1404Google Scholar
  21. 21.
    Khajeh M, Yamini Y, Sefidkon F, Bahramifar N (2004) Food Chem 86:587–591CrossRefGoogle Scholar
  22. 22.
    Albu S, Joyce E, Paniwnyk L, Lorimer JP, Mason TJ (2004) Ultrason Sonochem 11:261–265PubMedCrossRefGoogle Scholar
  23. 23.
    Pizzale L, Bortolomeazzi R, Vichy S, Überegger E, Conte LS (2002) J Sci Food Agric 82:1645–1651CrossRefGoogle Scholar
  24. 24.
    Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ (2003) Food Chem 83:255–262CrossRefGoogle Scholar
  25. 25.
    Kivilompolo M, Hyötyläinen T (2007) J Chromatogr A 1145:155–164Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Maarit Kivilompolo
    • 1
  • Vít Obůrka
    • 2
  • Tuulia Hyötyläinen
    • 1
  1. 1.Laboratory of Analytical Chemistry,Department of ChemistryUniversity of HelsinkiHelsinkiFinland
  2. 2.Faculty of ChemistryBrno University of TechnologyBrnoCzech Republic

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