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Analytical and Bioanalytical Chemistry

, Volume 388, Issue 1, pp 227–234 | Cite as

Molecularly imprinted polymer solid-phase extraction coupled to square wave voltammetry at carbon fibre microelectrodes for the determination of fenbendazole in beef liver

  • A. Guzmán-Vázquez de Prada
  • Oscar A. Loaiza
  • B. Serra
  • D. Morales
  • P. Martínez-Ruiz
  • A. J. Reviejo
  • J. M. PingarrónEmail author
Original Paper

Abstract

A molecularly imprinted polymer was developed and used for solid-phase extraction (MISPE) of the antihelmintic fenbendazole in beef liver samples. Detection of the analyte was accomplished using square wave voltammetry (SWV) at a cylindrical carbon fibre microelectrode (CFME). A mixture of MeOH/HAc (9:1) was employed both as eluent in the MISPE system and as working medium for electrochemical detection of fenbendazole. The limit of detection was 1.9 × 10−7 mol L−1 (57 μg L−1), which was appropriate for the determination of fenbendazole at the maximum residue level permitted by the European Commission (500 μg kg−1 in liver). Given that the SW voltammetric analysis could not be directly performed in the sample extract as a consequence of interference from some sample components, a sample clean-up with a MIP for selectively retaining fenbendazole was performed. The MIP was synthesized using a 1:8:22 template/methacrylic acid/ethylene glycol dimethacrylate ratio. A Britton–Robinson Buffer of pH 9.0 was selected for retaining fenbendazole in the MIP cartridges, and an eluent volume of 5.0 mL at a flow rate of 2.0 mL min−1 was chosen in the elution step. Cross-reactivity with the MIP was observed for other benzimidazoles. The synthesized MIP exhibited a good selectivity for benzimidazoles with respect to other veterinary drugs. The applicability of the MISPE-SWV method was tested with beef liver samples, spiked with fenbendazole at 5,000 and 500 μg kg−1. Results obtained for ten different liver samples yielded mean recoveries of (95 ± 12)% and (96 ± 11)% for the upper and lower concentration level, respectively.

Keywords

Benzimidazoles Fenbendazole Molecularly imprinted polymer Square wave voltammetry Beef liver 

Notes

Acknowledgements

Financial support from the Ministerio de Educación y Ciencia (Project BQU2003-00365) is gratefully acknowledged.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • A. Guzmán-Vázquez de Prada
    • 1
  • Oscar A. Loaiza
    • 1
  • B. Serra
    • 1
  • D. Morales
    • 1
  • P. Martínez-Ruiz
    • 2
  • A. J. Reviejo
    • 1
  • J. M. Pingarrón
    • 1
    Email author
  1. 1.Department of Analytical Chemistry, Faculty of ChemistryComplutense University of MadridMadridSpain
  2. 2.Department of Organic Chemistry, Faculty of ChemistryComplutense University of MadridMadridSpain

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