Analytical and Bioanalytical Chemistry

, Volume 387, Issue 4, pp 1561–1564 | Cite as

Time-resolved fluoroimmunoassay for ractopamine in swine tissue

  • Jianzhong Shen
  • Zhen Zhang
  • Yan Yao
  • Weimin Shi
  • Yibing Liu
  • Suxia Zhang
Original Paper

Abstract

This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC–ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1–10 μg kg−1, recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection limit was 0.1 μg kg−1. The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was confirmed by liquid chromatography–tandem mass spectrometry.

Keywords

Time-resolved fluoroimmunoassay Ractopamine Swine tissue 

Notes

Acknowledgement

This work was supported by the National Natural Science Foundation of China (NSFC) grant 30471305.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jianzhong Shen
    • 1
  • Zhen Zhang
    • 1
  • Yan Yao
    • 2
  • Weimin Shi
    • 1
  • Yibing Liu
    • 2
  • Suxia Zhang
    • 1
  1. 1.Department of Pharmacology and Toxicology, College of Veterinary MedicineChina Agricultural UniversityBeijingChina
  2. 2.Department of IsotopeChina Institute of Atomic EnergyBeijingChina

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