Time-resolved fluoroimmunoassay for ractopamine in swine tissue
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This study describes the development and validation of a time-resolved fluoroimmunoassay (TR-FIA) for screening ractopamine (RAC) in swine tissue. The method is based on the direct competitive-type immunoassay using europium-labeled anti-RAC monoclonal antibody as a tracer and RAC–ovalbumin as a solid-phase antigen. When RAC was spiked at levels of 1–10 μg kg−1, recoveries ranged from 88.2 to 118.5% for swine liver and muscle with coefficients of variation from 7.1 to 20.5%. The detection limit was 0.1 μg kg−1. The proposed TR-FIA method was applied to the determination of RAC in an actual residue study and the applicability was confirmed by liquid chromatography–tandem mass spectrometry.
KeywordsTime-resolved fluoroimmunoassay Ractopamine Swine tissue
This work was supported by the National Natural Science Foundation of China (NSFC) grant 30471305.
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