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Analytical and Bioanalytical Chemistry

, Volume 387, Issue 3, pp 1007–1016 | Cite as

Selective solid-phase extraction of tebuconazole in biological and environmental samples using molecularly imprinted polymers

  • Mai-ling Hu
  • Ming Jiang
  • Peng Wang
  • Su-rong Mei
  • Yan-fei Lin
  • Xiao-zhong Hu
  • Yun Shi
  • Bin LuEmail author
  • Kang Dai
Original Paper

Abstract

Molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization using tebuconazole (TBZ) as a template. Frontal chromatography and selectivity experiments were used to determine the binding capabilities and binding specificities of different MIPs. The polymer that had the highest binding selectivity and capability was used as the solid-phase extraction (SPE) sorbent for the direct extraction of TBZ from different biological and environmental samples (cabbage, pannage, shrimp, orange juice and tap water). The extraction protocol was optimized and the optimum conditions were: conditioning with 5 mL methanol:acetic acid (9:1), 5 mL methanol and 5 mL water respectively, loading with 5 mL aqueous samples, washing with 1.2 mL acetonitrile (ACN):phosphate buffer (5:5, pH3), and eluting with 3 mL methanol. The MIPs were able to selectively recognize, effectively trap and preconcentrate TBZ over a concentration range of 0.5–15 μmol/L. The intraday and interday RSDs were less than 9.7% and 8.6%, respectively. The limit of quantification was 0.1 μmol/L. Under optimum conditions, the MISPE recoveries of spiked cabbage, pannage, shrimp, orange juice and tap water were 62.3%, 75.8%, 71.6%, 89% and 93.9%, respectively. MISPE gave better HPLC separation efficiencies and higher recoveries than C18 SPE and strong cation exchange (SCX) SPE.

Figure

HPLC analysis of spiked pannage after MISPE (A) and after C18 SPE (B). HQ (1), E3 (2), p-NP (3), FTF (4), TBZ (5), PNZ (6), HXZ (7)

Keywords

Molecularly imprinted polymers Frontal chromatography Solid-phase extraction Tebuconazole Biological and environmental samples 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 20307004 and NO. 20477013), and the Entry-Exit Inspection and Quarantine Bureau of PRC (NO 2005G0082).

Supplementary material

216_2006_1004_MOESM1_ESM.doc (206 kb)
Fig. S1 Scanning electron micrographs of the particles prepared: a the length of the bar is 1 μm; b the length of the bar is 5 μm (DOC 210 kb)

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Mai-ling Hu
    • 1
  • Ming Jiang
    • 1
  • Peng Wang
    • 2
  • Su-rong Mei
    • 1
  • Yan-fei Lin
    • 2
  • Xiao-zhong Hu
    • 2
  • Yun Shi
    • 1
  • Bin Lu
    • 1
    Email author
  • Kang Dai
    • 1
  1. 1.Key Laboratory of Environment & Health of Ministry of Education, Institute of Environmental Medicine, School of Public Health, Tongji Medical CollegeHuazhong University of Science and TechnologyHubeiPeople’s Republic of China
  2. 2.Hubei Entry-Exit Inspection and Quarantine Bureau of PRCHubeiChina

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