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Analytical and Bioanalytical Chemistry

, Volume 387, Issue 2, pp 695–701 | Cite as

Determination of doxycycline in pharmaceuticals and human urine by micellar electrokinetic capillary chromatography

  • Rade Injac
  • Javor Kac
  • Samo Kreft
  • Borut Strukelj
Original Paper

Abstract

Micellar electrokinetic capillary chromatography (MEKC) was performed at 25 °C and 30 kV (under a pressure of 15 mbar), using 30 mM borate buffer containing 60 mM sodium dodecysulfate (SDS) and 5% (v/v) methanol as background electrolyte (pH 9.0) to determine doxycycline. UV detection was at 350 nm. The method was shown to be specific, accurate (recovery was 100.3 ± 1.0%), linear over the tested range (correlation coefficient 0.9995) and precise (RSD <1.9%). The method was used to determine doxycycline in tablets, capsules and human urine after oral application.

Keywords

Doxycycline Micellar electrokinetic capillary chromatography (MEKC) Pharmaceuticals Human urine 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Rade Injac
    • 1
  • Javor Kac
    • 1
  • Samo Kreft
    • 1
  • Borut Strukelj
    • 1
  1. 1.Institute of Pharmaceutical Biology, Faculty of PharmacyUniversity of LjubljanaLjubljanaSlovenia

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