Application of an in-line imprinted polymer column in a potentiometric flow-injection chemical sensor to the determination of the carbamate pesticide carbaryl in complex biological matrices
- 200 Downloads
A flow-injection biosensor-like system based on a nonenzymatic approach has been developed to determine the carbamate pesticide carbaryl in complex biological samples without lengthy and expensive extraction steps. Molecularly imprinted polymeric beads were used to immobilize carbaryl from biological samples. pH variation permitted the elution of carbaryl from the binding cavity to the flow cell. A pH electrode was used to detect changes in the charge of carbaryl in the sample solution resulting from the protonation and deprotonation of the molecule over different pH ranges. At pH 2.0, the secondary amine group is protonated, giving a (+1) charge to the carbaryl molecule. At pH 8.0, the ionized carbaryl loses a proton to become neutral, changing the local pH of the flow cell. The pH change at the flow cell generated by the deprotonation of carbaryl ion in alkaline medium was used to determine the carbaryl concentration. Parameters influencing the performance of the system were optimized for use in the detection procedure. The validated biosensor-like system had a carbaryl detection limit of 10.0 μg/mL and a response that was linear (r 2 > 0.98) over the concentration range of 10.0–00 μg/mL.
KeywordsPesticides Potentiometric Flow injection Biosensor-like Molecularly imprinted polymer Suspension polymerization Detection
This work was made possible by the support of Charles River Laboratories Preclinical Services Montreal, Inc. (CRM) and Concordia University.
- 1.Baron RL (1991) In: Hayes WH Jr, Laws ER Jr (eds) Handbook of pesticide toxicology. Academic, San Diego, CA, 3:1125–1189Google Scholar
- 3.Casale GP, Vennerstrom JL, Bavari S, Wang TL (1993) Immunopharmacol Immunotoxicol 15:199Google Scholar
- 4.US Department of Health and Human Services (1992) Hazardous substances databank (HSDB): entry for carbaryl. National Toxicology Information Program, National Library of Medicine, Bethesda, MD (available online at http://toxnet.nlm.nih.gov/cgi-bin/sis/search/f?./temp/~SdbQGC:1, last accessed 25th October 2006)
- 5.Mimmo DR, McEwen LC (1994) Pesticides. In: Calow P (ed) Handbook of ecotoxicology, vol 2. Blackwell, Oxford, pp 155–203Google Scholar
- 9.Rekha K, Thakur MS, Karanth NG (2000) Crit Rev Biotechnol 20(3):213–235Google Scholar
- 12.Jungbluth F (1996) Crop protection policy in Thailand: economic and political factors influencing pesticide use (Pesticide Policy Project Publ Ser No 5). GTZ/University of Hannover, HannoverGoogle Scholar
- 15.US FDA (2001) Guidance for industry—bioanalytical method validation. US Food and Drug Administration, Rockville, MDGoogle Scholar