Analytical and Bioanalytical Chemistry

, Volume 387, Issue 5, pp 1779–1789 | Cite as

A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions

  • Adriana Szeghalmi
  • Susan Kaminskyj
  • Kathleen M. Gough
Original Paper

Abstract

Synchrotron FTIR can provide high spatial resolution (<10 μm pixel size) in situ biochemical analyses of intact biotissues, an area of increasing importance in the post-genomic era, as gene functions and gene networks are coming under direct scrutiny. With this technique, we can simultaneously assess multiple aspects of cell biochemistry and cytoplasmic composition. In this paper, we report the first results of our synchrotron FTIR examination of hyphae of three important fungal model systems, each with sequenced genomes and a wealth of research: Aspergillus, Neurospora, and Rhizopus. We have analyzed the FTIR maps of Aspergillus nidulans cells containing the hypA1 allele, a well-characterized single-gene temperature-sensitive morphogenetic mutation. The hypA1 cells resemble wildtype at 28 °C but have growth defects at 42 °C. We have also investigated Neurospora and Rhizopus cultures grown in media with optimal or elevated pH. Significant differences between the spectra of the three fungi are likely related to differences in composition and structure. In addition, high spatial resolution synchrotron FTIR spectroscopy provides an outstanding method for monitoring subtle subcellular changes that accompany environmental stress.

Figure

Photomicrographs and FTIR spectra acquired along Rhizopus hyphae grown at pH 6.5 (a) and pH 8.5 (b). Scale bars 50 μm

Keywords

Synchrotron FTIR spectromicroscopy Aspergillus nidulans Neurospora Rhizopus Fungal tip growth under stress 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Adriana Szeghalmi
    • 1
  • Susan Kaminskyj
    • 2
  • Kathleen M. Gough
    • 1
  1. 1.Department of ChemistryUniversity of ManitobaWinnipegCanada
  2. 2.Department of BiologyUniversity of SaskatchewanSaskatoonCanada

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