Analytical and Bioanalytical Chemistry

, Volume 387, Issue 5, pp 1643–1648

Study of normal colorectal tissue by FT-Raman spectroscopy

  • P. O. Andrade
  • R. A. Bitar
  • K. Yassoyama
  • H. Martinho
  • A. M. E. Santo
  • P. M. Bruno
  • A. A. Martin
Original Paper

Abstract

FT-Raman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum. The samples were collected from 39 patients, providing 144 spectra for the statistical analysis. The results enable one to estabilish three well-defined spectroscopic groups of non-altered coloretal tissues that were consistently checked by statistical (clustering) and biological (histopathology) analyses: group 1 is represented by samples with the presence of epithelial layer, connective tissue papillae, and smooth muscle tissue; group 2 comprises tissues with epithelial layer and connective tissue papillae; group 3 presented mostly fatty and slack conjunctive tissue. The study reveals the existence of an intrinsic spectral variability for each patient that must be considered when sampling tissues fragments to build a spectral database. This is the first step for future studies and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancer.

Keywords

FT-Raman spectroscopy Colorectal Cancer Optical biopsy 

References

  1. 1.
    Libutti SK et al (2005) Cancer of the colon. In: VT DeVita Jr et al (eds) Cancer principles and practice of oncology, 7th edn. Lippincott Williams and Wilkins, Philadelphia, pp 1061–1109Google Scholar
  2. 2.
    Stewart BW, Kleihues P (eds) (2003) World cancer report. World Health Organization, International Agency for Research on CancerGoogle Scholar
  3. 3.
    INCA (2006) Ministerio da Saúde, Secretaria Nacional de Assistência à Saúde. Instituto Nacional do Câncer. Câncer de Colo-retal. Avaiable at http://www.inca.org.br. Accessed on 28 April 2006
  4. 4.
    Vernon SW (1997) J Nat Cancer Inst 89:1406CrossRefGoogle Scholar
  5. 5.
    Mahadevan-Jansen A, Richards-Kortum R (1996) J Biom Opt 1(1):31CrossRefGoogle Scholar
  6. 6.
    Hanlon EB, Manoharan R, Koo TW, Shafer KE, Motz JT, Fitzmaurice M, Kramer JR, Itzkan I, Dasari RR, Feld MS (2000) Phys Med Biol 45:R1–R59CrossRefGoogle Scholar
  7. 7.
    Stone S, Kendall C, Smith J, Crow P, Barr H (2003) Raman spectroscopy for identification of epithelial cancers. Farady Discuss 126:141–157CrossRefGoogle Scholar
  8. 8.
    Yamasaki H, Kaminaka S, Kohda E, Mukai M, Hamagushi H (2003) The diagnosis of lung cancer using 1064-nm excited near-infrared multichannel Raman spectroscopy. Radiat Med 21(1):1–6Google Scholar
  9. 9.
    Baena JR, Lendi B (2004) Raman spectroscopy in chemical bioanalysis. Curr Opin Chem Biol 8:534–539CrossRefGoogle Scholar
  10. 10.
    Choo-Smith LP, Edwards HGM, Heule F, Barr H et al (2002) Medical applications of Raman spectroscopy: from proof of principle to clinical implementation. Biopolymers(Biospectroscopy) 67:1–9Google Scholar
  11. 11.
    Bitar RA, Martinho SH, Criollo TJC, Ramalho ZNL, Netto MM, Martin AA (2006) Biochemical analysis of human breast tissues using FT-Raman spectroscopy. J Biomed Opt (in press) (q-bio.TO/0603037)Google Scholar
  12. 12.
    Montgomery E, Bronner MP, Goldblum JR, Greenson JK, Haber HM, Hart J, Lamps LW, Lauwers GY, Lazenby AJ, Lewin DN, Robert ME, Toledanao AY, Washington K (2001) Hum Pathol 32:268–378Google Scholar
  13. 13.
    Huang Z, Zheng W, Widjaja E, Mo J, Sheppard C (2006) Proc of SPIE:60930Q1–4Google Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • P. O. Andrade
    • 1
  • R. A. Bitar
    • 1
  • K. Yassoyama
    • 1
  • H. Martinho
    • 1
  • A. M. E. Santo
    • 1
  • P. M. Bruno
    • 2
  • A. A. Martin
    • 1
  1. 1.Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development (IP&D)Universidade do Vale do Paraiba (UniVap)São José dos Campos, SPBrazil
  2. 2.Vivalle HospitalSão José dos Campos, SPBrazil

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