Imaging molecular events in single living cells
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Fluorescence imaging could be the most powerful technique available for observing spatial and temporal dynamics of biomolecules in living cells, if fluorescent indicators for the relevant biomolecules become available. We have recently developed fluorescent indicators for a variety of second messengers or protein phosphorylations. Using the indicators, we have visualized spatial and temporal dynamics of these molecular events in single living cells. These fluorescent indicators are becoming an indispensable tool for understanding the complex mechanism of signal transduction in living cells.
KeywordsFluorescent indicator Fluorescence resonance energy transfer Second messenger Protein phosphorylation Nuclear receptor ligand
This work has been supported by JST Japan Science and Technology Agency and grants from the Ministry of Education, Science, and Culture of Japan, the Takeda Science Foundation, and the Sumitomo Foundation.
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