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Analytical and Bioanalytical Chemistry

, Volume 385, Issue 8, pp 1575–1579 | Cite as

Rapid analysis of native neomycin components on a portable capillary electrophoresis system with potential gradient detection

  • LingLing Yuan
  • HongPing Wei
  • HuaTao Feng
  • Sam F. Y. LiEmail author
Technical Note

Abstract

A simple method based on capillary electrophoresis with potential gradient detection was developed to separate and detect neomycin components within 4 min without a derivatization step. Satisfactory separation and good repeatability were obtained using a separation buffer composed of 1 mM ammonium citrate (pH 3.5). The linearity of the method ranged from 10 to 1000 ppm with a limit of detection for neomycin B of about 7 ppm. After a simple dilution and filtering pretreatment step, neomycin components in three real samples were successfully analyzed without any major interference. Due to its simplicity and reliability, this method could provide an excellent alternative to the assays currently listed in U.S. and European Pharmacopoeia. The experiments were performed on a portable capillary electrophoresis system and, hence, the method can be readily applied to field analysis and point-of-care testing.

Figure

Photo of portable CE-P2-PGD system

Keywords

Aminoglycoside antibiotics Capillary electrophoresis Neomycin Potential gradient detection 

Abbreviations

CTAB

Cetyltrimethylammonium bromide

MAA

Mercaptoacetic acid

OPA

1,2-Phthalic dicarboxaldehyde

PGD

Potential gradient detection

UV

Ultraviolet

Notes

Acknowledgements

The authors would like to thank the National University of Singapore for the financial support given to this research, and CE Resources Pte Ltd for extending us the use of the CE-P2 Capillary Electrophoresis System used in this work.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • LingLing Yuan
    • 1
  • HongPing Wei
    • 2
  • HuaTao Feng
    • 1
  • Sam F. Y. Li
    • 1
    Email author
  1. 1.Department of ChemistryNational University of SingaporeSingaporeRepublic of Singapore
  2. 2.The Key Laboratory of Biomedical Photonics of Ministry of EducationHuazhong University of Science and TechnologyWuhanPeople’s Republic of China

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