Effective PCR detection of animal species in highly processed animal byproducts and compound feeds

  • Olivier Fumière
  • Marc Dubois
  • Vincent Baeten
  • Christoph von Holst
  • Gilbert Berben
Original Paper

Abstract

In this paper we present a polymerase chain reaction (PCR)-based method for detecting meat and bone meal (MBM) in compound feedingstuffs. By choosing adequate DNA targets from an appropriate localisation in the genome, the real-time PCR method developed here proved to be robust to severe heat treatment of the MBM, showing high sensitivity in the detection of MBM. The method developed here permits the specific detection of processed pig and cattle materials treated at 134 °C in various feed matrices down to a limit of detection of about 0.1%. This technique has also been successfully applied to well-characterised MBM samples heated to as high as 141 °C, as well as to various blind feed samples with very low MBM contents. Finally, the method also passed several official European ring trials.

Keywords

Feed Meat and bone meal (MBM) BSE Polymerase chain reaction Real time PCR Mitochondrial DNA Species identification Pig Cattle 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Olivier Fumière
    • 1
  • Marc Dubois
    • 1
  • Vincent Baeten
    • 1
  • Christoph von Holst
    • 2
  • Gilbert Berben
    • 1
  1. 1.Département Qualité des Productions AgricolesCentre Wallon de Recherches agronomiques (CRA-W)GemblouxBelgium
  2. 2.European Commission, Directorate-General Joint Research CentreInstitute for Reference Materials and Measurments (JRC-IRMM)GeelBelgium

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