Analytical and Bioanalytical Chemistry

, Volume 386, Issue 3, pp 416–426 | Cite as

Recent developments in microarray-based enzyme assays: from functional annotation to substrate/inhibitor fingerprinting

  • Hongyan Sun
  • Souvik Chattopadhaya
  • Jun Wang
  • Shao Q. Yao
Review

Abstract

Recent advances in proteomics have provided impetus towards the development of robust technologies for high-throughput studies of enzymes. The term “catalomics” defines an emerging ‘-omics’ field in which high-throughput studies of enzymes are carried out by using advanced chemical proteomics approaches. Of the various available methods, microarrays have emerged as a powerful and versatile platform to accelerate not only the functional annotation but also the substrate and inhibitor specificity (e.g. substrate and inhibitor fingerprinting, respectively) of enzymes. Herein, we review recent developments in the fabrication of various types of microarray technologies (protein-, peptide- and small-molecule-based microarrays) and their applications in high-throughput characterizations of enzymes.

Keywords

Enzyme assay Microarray Catalomics Activity-based probe Activity-based fingerprinting Inhibitor fingerprinting Drug discovery 

Notes

Acknowledgements

Funding support was provided by the National University of Singapore (NUS) and the Agency for Science, Technology and Research (A*STAR) of Singapore.

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Hongyan Sun
    • 1
  • Souvik Chattopadhaya
    • 2
  • Jun Wang
    • 1
  • Shao Q. Yao
    • 1
    • 2
    • 3
  1. 1.Department of ChemistryNational University of SingaporeSingaporeSingapore
  2. 2.Department of Biological SciencesNational University of SingaporeSingaporeSingapore
  3. 3.Medicinal Chemistry Program of the Office of Life SciencesNational University of SingaporeSingaporeSingapore

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