Analytical and Bioanalytical Chemistry

, Volume 386, Issue 3, pp 627–632 | Cite as

Longitudinal diffusion behavior of hemicyanine dyes across phospholipid vesicle membranes as studied by second-harmonic generation and fluorescence spectroscopies

  • Akira Yamaguchi
  • Masaki Nakano
  • Kimihisa Nochi
  • Tomohisa Yamashita
  • Kotaro Morita
  • Norio Teramae
Original Paper

Abstract

The adsorption and longitudinal diffusion behaviors of a series of hemicyanine dyes to phospholipid vesicle membranes were studied by second-harmonic generation (SHG) and fluorescence spectroscopies. It was observed that the longitudinal diffusion of cationic hemicyanine dyes takes place immediately after the initial adsorption of these dyes to the outer surface of the vesicle membrane. In contrast, hardly any amount of a zwitterionic hemicyanine dye with a sulfonate group diffused across the vesicle membrane within the measurement time (<2000 s). Based on the difference in the time-course responses of SHG and fluorescence spectroscopies for all of the hemicyanine dyes tested, we propose that hydration of the sulfonate group is mainly responsible for the low diffusivity of the zwitterionic hemicyanine dye.

Keywords

Second-harmonic generation Fluorescence Phospholipid vesicle Longitudinal diffusion 

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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Akira Yamaguchi
    • 1
    • 2
  • Masaki Nakano
    • 1
  • Kimihisa Nochi
    • 1
  • Tomohisa Yamashita
    • 1
  • Kotaro Morita
    • 1
  • Norio Teramae
    • 1
  1. 1.Department of Chemistry, Graduate School of ScienceTohoku UniversityAoba-ku, SendaiJapan
  2. 2.PRESTOJapan Science and Technology AgencyKawaguchiJapan

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