Analytical and Bioanalytical Chemistry

, Volume 385, Issue 1, pp 128–133 | Cite as

Chemiluminescence determination of metformin based on hydroxyl radical reaction and molecularly imprinted polymer on-line enrichment

  • Chao He
  • Zhujun Zhang
  • Deyong He
  • Yan Xiong
Original Paper


A novel and simple chemiluminescence (CL) method has been developed and validated for determination of metformin. This method is based on hydroxyl radical chemiluminescence—the hydroxyl radical generated by reaction of Cu(II) and hydrogen peroxide oxidizes rhodamine B (RhB) to produce weak CL which can be enhanced by metformin. At the same time, metformin molecularly imprinted polymer (MIP) was synthesized. After enrichment based on the selectivity of metformin-MIP, the CL method was successfully applied to the determination of metformin in human serum. The linear range was from 1.0×10−8 to 1.0×10−6 g mL−1 and the detection limit was 4×10−9 g mL−1. The relative standard deviation at 2.0×10−7 g mL−1 by use of MIP was 3.67% (n=7).


Flow injection Chemiluminescence Hydroxyl radical Metformin Molecularly imprinted polymer 



This study was supported by the Natural Science Foundation of China (No. 30470886).


  1. 1.
    Wang ZP, Zhang ZJ, Fu ZF, Xiong Y, Zhang X (2003) Anal Biochem Chem 377:660–665Google Scholar
  2. 2.
    Hsu SC, Don TM, Chiu WY (2002) Polym Degrad Stab 75:73–83CrossRefGoogle Scholar
  3. 3.
    Li H, Wang QJ (2004) Anal Biochem Chem 378:1801–1805CrossRefGoogle Scholar
  4. 4.
    Mujahid A, Yoshiki Y, Akiba Y, Toyomizu M (2005) Poultry Sci 84:307–314Google Scholar
  5. 5.
    Kawatani T, Lin JM, Yamada M (2000) Analyst 125:2075–2078CrossRefGoogle Scholar
  6. 6.
    Cheng ZY, Yan GT, Li YZ, Chang WB (2003) Anal Biochem Chem 375:376–380Google Scholar
  7. 7.
    Luo Y, Kustin K, Epstein IR Inorg (1988) Chem 27:2489–2496Google Scholar
  8. 8.
    El-Khateeb SZ, Assaad HN, El-Bardicy MG, Ahmad AS (1988) Anal Chim Acta 208:321–324CrossRefGoogle Scholar
  9. 9.
    Hassan SSM, Mahmoud WH, Elmosallamy MAF, Othman AHM (1999) Anal Chim Acta 378:299–311CrossRefGoogle Scholar
  10. 10.
    Habib IHI, Kamel MS (2003) Talanta 60:185–190CrossRefGoogle Scholar
  11. 11.
    Amini H, Ahmadiani A, Gazerani P (2005) J Chromatogr B 824:319–322Google Scholar
  12. 12.
    Wang YW, Tang YB, Gu JK, Fawcett JP, Bai X (2004) J Chromatogr B 808:215–219CrossRefGoogle Scholar
  13. 13.
    Yardýmc C, Özaltýn N (2005) Anal Chim Acta 549:88–95CrossRefGoogle Scholar
  14. 14.
    Wang ZP, Zhang ZJ, Fu ZF, Luo WF, Zhang X (2003) Anal Lett 36:2683–2697CrossRefGoogle Scholar
  15. 15.
    Kempe M (1996) Anal Chem 68:1948–1953CrossRefGoogle Scholar
  16. 16.
    Brüggemann O, Freitag R, Whitcombe MJ, Vulfson EN (1997) J Chromatogr A 781:43–53CrossRefGoogle Scholar
  17. 17.
    Peng H, Liang CD, He DL, Nie LH, Yao SZ (2000) Talanta 52:441–448CrossRefGoogle Scholar
  18. 18.
    Burow M, Minoura N (1996) Biochem Biophys Res Commun 227:419–422CrossRefGoogle Scholar
  19. 19.
    Zhou HJ, Zhang ZJ, He DY, Hu YF, Huang Y, Chen DL (2004) Anal Chim Acta 523:237–242CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.Institute of Analytical Science, The Key Lab of Analytical Chemistry of ChongqingSouthwest Normal UniversityBeibeiP.R. China

Personalised recommendations