Analytical and Bioanalytical Chemistry

, Volume 385, Issue 1, pp 153–160 | Cite as

Investigating the post-chemiluminescence behavior of phenothiazine medications in the luminol–potassium ferricyanide system: molecular imprinting–post-chemiluminescence method for the determination of chlorpromazine hydrochloride

  • Weifen Niu
  • Na Feng
  • Fei Nie
  • Jiuru LuEmail author
Original Paper


A new post-chemiluminescence (PCL) phenomenon was observed when phenothiazine medications were injected into the reaction mixture after the chemiluminescence (CL) reaction of luminol and potassium ferricyanide had finished. A possible reaction mechanism was proposed based on studies of the kinetic characteristics of the CL, CL spectra, fluorescence spectra, and on other experiments. The feasibility of determining various phenothiazine medications by utilizing these PCL reactions was examined. A molecular imprinting–post-chemiluminescence (MI-PCL) method was established for the determination of chlorpromazine hydrochloride using a chlorpromazine hydrochloride-imprinted polymer (MIP) as the recognition material. The method displayed high selectivity and high sensitivity. The linear range of the method was 1.0×10−8∼1.0×10−6, with a linear correlation coefficient of 0.9985. The detection limit was 3×10−9 g/ml chlorpromazine hydrochloride, and the relative standard deviation for a 1.0×10−7 g/ml chlorpromazine hydrochloride solution was 4.0% (n=11). The method has been applied to the determination of chlorpromazine hydrochloride in urine and animal drinking water with satisfactory results.


Post-chemiluminescence (PCL) Molecular imprinted polymer (MIP) Phenothiazine medications Chlorpromazine hydrochloride 



The authors gratefully acknowledge financial support from the National Natural Science Foundation of China (Grant No. 20275023) and the Natural Science Foundation of Shaanxi Province (Grant No. 2002B12).


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Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  1. 1.School of Chemistry and Materials ScienceShaanxi Normal UniversityXi’anP.R. China

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