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Analytical and Bioanalytical Chemistry

, Volume 383, Issue 3, pp 454–460 | Cite as

Determination of arsenic species and arsenosugars in marine samples by HPLC–ICP–MS

  • Shizuko HirataEmail author
  • Hideki Toshimitsu
Special Issue Paper

Abstract

Arsenic-speciation analysis in marine samples was performed by high-pressure liquid chromatography (HPLC) with ICP–MS detection. Separation of eight arsenic species—AsIII, MMA, DMA, AsV, AB, TMAO, AC and TeMAs+—was achieved on a C18 column with isocratic elution (pH 3.0), under which conditions AsIII and MMA co-eluted. The entire separation was accomplished in 15 min. The HPLC–ICP–MS detection limits for the eight arsenic species were in the range 0.03–0.23 μg L−1 based on 3σ for the blank response (n=5). The precision was calculated to be 2.4–8.0% (RSD) for the eight species. The method was successfully applied to several marine samples, e.g. oysters, fish, shrimps, and marine algae. Low-power microwave digestion was employed for extraction of arsenic from seafood products; ultrasonic extraction was employed for the extraction of arsenic from seaweeds. Separation of arsenosugars was achieved on an anion-exchange column. Concentrations of arsenosugars 2, 3, and 4 in marine algae were in the range 0.18–9.59 μg g−1.

Keywords

HPLC–ICP–MS Arsenic species Arsenosugars Marine algae Microwave extraction Sonication 

Notes

Acknowledgements

We are grateful to Professor K.V. Francesconi of Graz University in Austria for providing us a Fucus sample, which is useful for evaluating arsenosugars.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.National Institute of Advanced Industrial Science and Technology (AIST)KureJapan
  2. 2.Graduate School of Kinki UniversityHigashi-HiroshimaJapan

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