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Analytical and Bioanalytical Chemistry

, Volume 382, Issue 1, pp 51–58 | Cite as

Molecularly imprinted polymers as tools for the screening of felodipine from dihydropyridine calcium antagonists by pressurized capillary electrochromatography

  • Qiliang Deng
  • Zhihong Lun
  • Hua Shao
  • Chao Yan
  • Ruyu GaoEmail author
Original Paper

Abstract

A group of structurally similar dihydropyridine calcium antagonists (DHPs) and related compounds were used to simulate a combinatorial library. A molecularly imprinted polymer (MIP) comprising felodipine (FLD) was synthesized in situ inside the capillary for use in the separation of FLD from other DHPs by pressurized electrochromatography (pCEC). To evaluate the feasibility of using the MIP columns for the separation of FLD, parameters including pH, the applied voltages, and the effect of organic modifier were studied. The results indicated that the MIP columns demonstrated better recognition properties over a pH range of 4–6. The efficiency (plates/m) at pH 5.0 for the non-imprinted analytes was 117,000 for thiourea, 18,700 for nicarpidine, 17,300 for nisoldipine, and 14,600 for nifedipine; however, the efficiency for the imprinted analyte FLD was low, as evidenced by the broad peak, yielding only 5,100 plates/m. The column efficiency was also investigated under both micro-HPLC and pCEC conditions.

Keywords

Pressurized capillary electrochromatography Dihydropyridine calcium antagonists Molecularly imprinted monolithic column 

Notes

Acknowledgments

Financial support of the research by a grant from the National Nature Science Foundation of China (No. 20175010) is gratefully acknowledged.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Qiliang Deng
    • 1
  • Zhihong Lun
    • 1
  • Hua Shao
    • 1
  • Chao Yan
    • 2
  • Ruyu Gao
    • 1
    Email author
  1. 1.State Key Laboratory of Elemento-Organic ChemistryNankai UniversityTianjinPR China
  2. 2.Unimicro Technologies Inc.PleasantonUSA

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