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Analytical and Bioanalytical Chemistry

, Volume 381, Issue 8, pp 1596–1603 | Cite as

Flow-injection technique for determination of uranium and thorium isotopes in urine by inductively coupled plasma mass spectrometry

  • Karima Benkhedda
  • Vladimir N. Epov
  • R. Douglas Evans
Original Paper

Abstract

A sensitive and efficient flow-injection (FI) preconcentration and matrix-separation technique coupled to sector field ICP–mass spectrometry (SF-ICP–MS) has been developed and validated for simultaneous determination of ultra-low levels of uranium (U) and thorium (Th) in human urine. The method is based on selective retention of U and Th from a urine matrix, after microwave digestion, on an extraction chromatographic TRU resin, as an alternative to U/TEVA resin, and their subsequent elution with ammonium oxalate. Using a 10 mL sample, the limits of detection achieved for 238U and 232Th were 0.02 and 0.03 ng L−1, respectively. The accuracy of the method was checked by spike-recovery measurements. Levels of U and Th in human urine were found to be in the ranges 1.86–5.50 and 0.176–2.35 ng L−1, respectively, well in agreement with levels considered normal for non-occupationally exposed persons. The precision obtained for five replicate measurements of a urine sample was 2 and 3% for U and Th, respectively. The method also enables on-line measurements of the 235U/238U isotope ratios in urine. Precision of 0.82–1.04% (RSD) was obtained for 235U/238U at low ng L−1 levels, using the FI transient signal approach.

Keywords

Uranium Thorium Flow injection Urine SF-ICP–MS Isotope ratios 

Notes

Acknowledgement

The authors acknowledge CBRN Research and Technology Initiative (CRTI) and the National Sciences and Engineering Research Council of Canada (NSERC) for the financial support. We also would like to thank D. Brownell for the technical assistance and all the volunteers for their kind cooperation.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Karima Benkhedda
    • 1
    • 3
  • Vladimir N. Epov
    • 1
    • 2
  • R. Douglas Evans
    • 1
  1. 1.Environmental Resources StudiesTrent UniversityPeterboroughCanada
  2. 2.Vinogradov Institute of Geochemistry SB RASIrkutskRussia
  3. 3.Nutrition Research Division, 2203C Banting Research CentreHealth CanadaOttawaCanada

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