Analytical and Bioanalytical Chemistry

, Volume 381, Issue 8, pp 1592–1595 | Cite as

Determination of ochratoxin A in wine by liquid chromatography tandem mass spectrometry after combined anion-exchange/reversed-phase clean-up

Original Paper

Abstract

Ochratoxin A (OTA) is a mycotoxin produced by Aspergillus ochraceus and Penicillium verrucosum. It has been found and analysed in several foods and feeds. Owing to its toxicity and occurrence in food and feed, the European Community has issued directives and some countries have their own regulations for OTA contents in food, feed and beverages. This work describes a method for the determination of OTA in mulled and red wine. It is based on combined anion exchange/reversed-phase clean-up and was analysed by liquid chromatography coupled with tandem mass spectrometry (multiple reaction monitoring). The method was validated with natural contaminated and spiked wine samples with OTA contents from 1.34 to 3.48 μg kg−1. Owing to its accuracy, good reproducibility and repeatability, this easy method is a good alternative to liquid chromatography–fluorescence detection methods.

Keywords

Ochratoxin A Wine Tandem mass spectrometry Solid-phase extraction Anion exchange Liquid chromatography 

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.Department of Analytical ChemistryFederal Institute for Materials Research and TestingBerlinGermany

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