Analytical and Bioanalytical Chemistry

, Volume 384, Issue 1, pp 225–230 | Cite as

Synthesis of polyacrylamide gel beads with electrostatic functional groups for the molecular imprinting of bovine serum albumin

  • Xingshou Pang
  • Guoxiang ChengEmail author
  • Shulai Lu
  • Erjun Tang
Original Paper


Synthetic materials capable of recognizing proteins are important in separation, biosensors and biomaterials. In this study, bovine serum albumin-imprinted soft-wet polyacrylamide gel beads were prepared via inverse-phase suspension polymerization, using acrylamide and N,N′-methylene diacrylamide as polymeric matrix components and methacrylic acid as functional monomer. The adsorption study showed, through the imprinting process, that the imprinted gel beads had much higher adsorption capacity than the nonimprinted gel beads, and that the matching of the surface zeta-potential between the templates and the imprinted gel beads can enhance the imprinting effect. Adsorption kinetics indicated that the adsorption process could be described as an apparent first-order kinetic process for the gel beads. From the adsorption isotherm curve, we found that the adsorption of the imprinted gel beads was in agreement with the Langmuir adsorption model. Moreover, selectivity testing of the imprinted gel beads showed that imprinted gel beads exhibited good recognition for BSA as compared to the control protein. We speculate that the formation of complementary shapes and multiple-point electrostatic interactions between the imprinting cavities and the template proteins are the two factors that lead to the imprinting effect.


Polyacrylamide gel beads Bovine serum albumin Molecular imprinting Methacrylic acid 



The authors would like to thank the National Natural Science Foundation of China (grant number: 50373032) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE, P.R.C. (2002–123) for supporting this research work.


  1. 1.
    Cormack PAG, Mosbach K (1999) React Funct Polym 41:115–124CrossRefGoogle Scholar
  2. 2.
    Wulff G (1995) Angew Chem Int Edit 31:1812–1832Google Scholar
  3. 3.
    Ansell RJ, Mosbach KJ (1997) J Chromatogr A 787:55–66Google Scholar
  4. 4.
    Chen W, Liu F, Xu Y, Li KA (2001) Anal Chim Acta 432: 277–282CrossRefGoogle Scholar
  5. 5.
    Cheong SH, Mcniven S, Uezu K, Goto M, Furusaki S (1997) Macromolecules 30: 1317–1322CrossRefGoogle Scholar
  6. 6.
    Lu SL, Cheng GX, Pang XS (2003) J Appl Polym Sci 89:3790–3796CrossRefGoogle Scholar
  7. 7.
    Zhang LY, Cheng GX, Fu C (2003) React Funct Polym 56:167–173CrossRefGoogle Scholar
  8. 8.
    Kempe M, Glad M, Mosbach K (1995) J Mol Recognit 8:35Google Scholar
  9. 9.
    Hjerten S, Liao J-L, Nakazato K, Wang Y, Zamaratskaina G, Zhang H-Y (1997) Chromatographia 44: 227–234CrossRefGoogle Scholar
  10. 10.
    Hirayama K, Burow M, Morikawa K, Minoura N (1998) Chem Lett 731Google Scholar
  11. 11.
    Shi HQ, Tsai W, Garrison MD, Ferrari S, Ratner BD (1999) Nature 398:593–597CrossRefPubMedGoogle Scholar
  12. 12.
    Ou SH, Wu MC, Chou TC, Liu CC (2004) Anal Chim Acta 504:163–166CrossRefGoogle Scholar
  13. 13.
    Pang XS, Cheng GX, Li RS, Lu SL, Zhang YH (2005) Anal Chim Acta 550:13–17CrossRefGoogle Scholar
  14. 14.
    Piletsky SA, Andersson HS, Nicholls IA (1999) Macromolecules 32:633–636CrossRefGoogle Scholar
  15. 15.
    Yin G, Liu Z, Zhan J, Ding FX, Yuan NJ (2002) Chem Eng J 87:181–186CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Xingshou Pang
    • 1
  • Guoxiang Cheng
    • 1
    Email author
  • Shulai Lu
    • 1
  • Erjun Tang
    • 1
  1. 1.School of Materials Science and EngineeringTianjin UniversityTianjinPR China

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