Interfacing liquid chromatography with atmospheric pressure MALDI-MS
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Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3 fmol using a solution of 50 nM peptide with 25 mM α-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500 Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method.
KeywordsMatrix assisted laser desorption/ionization IR ionization from solution IR matrix assisted laser desorption/ionization Liquid matrix Atmospheric pressure matrix assisted laser desorption/ionization
We thank SESI (Columbia, MD, USA) and its team for the loan and support of the IR laser and JDS Uniphase and GMP SA for the loan of the Power Chip laser.
Partial financial support from the Swiss National Science Foundation (grant no. 200020-103616) is gratefully acknowledged.
- 27.Laiko VV, Tan PV, Taranenko NI, Yakshin MA, Prasad CR, Doroshenko VM (2003) Proceedings of the 51st ASMS conference on mass spectrometry and allied topics, MontrealGoogle Scholar