Analytical and Bioanalytical Chemistry

, Volume 383, Issue 6, pp 895–902 | Cite as

Interfacing liquid chromatography with atmospheric pressure MALDI-MS

  • Jürg M. Daniel
  • Victor V. Laiko
  • Vladimir M. Doroshenko
  • Renato Zenobi
Original Paper


Two different strategies for coupling liquid chromatography with atmospheric pressure matrix assisted laser desorption/ionization (AP MALDI) are presented. The first method is flow-injection liquid AP UV-MALDI. Compared with previous similar research, the detection limit was improved 10 times to 8.3 fmol using a solution of 50 nM peptide with 25 mM α-cyano-4-hydroxycinnamic acid. The applicability of this method to measure oligosaccharides, actinomycin antibiotics, antibiotics, phosphopeptides, and proteins is demonstrated. The upper mass limit achieved with the current instrumentation is 6,500 Da (doubly charged cytochrome c). The feasibility of a second strategy based on single-droplet IR AP MALDI is demonstrated here. Aqueous peptide solutions were successfully measured by this method.


Matrix assisted laser desorption/ionization IR ionization from solution IR matrix assisted laser desorption/ionization Liquid matrix Atmospheric pressure matrix assisted laser desorption/ionization 



We thank SESI (Columbia, MD, USA) and its team for the loan and support of the IR laser and JDS Uniphase and GMP SA for the loan of the Power Chip laser.

Partial financial support from the Swiss National Science Foundation (grant no. 200020-103616) is gratefully acknowledged.


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Jürg M. Daniel
    • 1
  • Victor V. Laiko
    • 2
  • Vladimir M. Doroshenko
    • 2
  • Renato Zenobi
    • 1
  1. 1.Department of Chemistry and Applied BiosciencesSwiss Federal Institute of TechnologyZürichSwitzerland
  2. 2.MassTech Inc.ColumbiaUSA

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