Analytical and Bioanalytical Chemistry

, Volume 383, Issue 4, pp 668–672 | Cite as

An on-membrane quantitative analysis system for glycyrrhizin in licorice roots and traditional Chinese medicines

  • Osamu Morinaga
  • Ayako Fujino
  • Hiroyuki Tanaka
  • Yukihiro Shoyama
Original Paper


An on-membrane quantitative analysis system has been developed for determining glycyrrhizin (GC) in licorice roots and traditional Chinese medicines. A GC standard and the extracts of licorice roots and traditional Chinese medicines were applied to a polyethersulfone (PES) membrane and were developed by acetonitrile/water/formic acid (45:55:2, by volume), then treated with a NaIO4 solution followed by bovine serum albumin (BSA), resulting in a GC-BSA conjugate on a PES membrane. Anti-GC monoclonal antibody was bound and then a second antibody labeled with peroxidase directed against the first antibody. Finally a substrate reacted with the enzyme and gave staining. The stained membrane was scanned and coloring spots were analyzed quantitatively using graphic analysis by NIH Image software, indicating at least 0.5 μg of GC was clearly detectable. GC can be analyzed quantitatively between 1.0 and 8.0 μg.


Glycyrrhizin Eastern blotting NIH Image software Quantitative analysis 


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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Osamu Morinaga
    • 1
  • Ayako Fujino
    • 1
  • Hiroyuki Tanaka
    • 2
  • Yukihiro Shoyama
    • 2
  1. 1.Innovation Plaza FukuokaJapan Science and Technology AgencyFukuokaJapan
  2. 2.Department of Pharmacognosy, Graduate School of Pharmaceutical SciencesKyushu UniversityFukuokaJapan

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