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Analytical and Bioanalytical Chemistry

, Volume 383, Issue 4, pp 645–650 | Cite as

Determination of ropivacaine in human plasma using highly selective molecular imprint-based solid phase extraction and fast LC–MS analysis

  • Zoe Cobb
  • Lars I. AnderssonEmail author
Original Paper

Abstract

A method for determining ropivacaine in human plasma using highly selective molecular imprint-based solid phase extraction and LC–MS analysis was developed. The imprinted extraction material was prepared using a structural analogue of ropivacaine as the template. The efficient sample cleanup achieved allowed single MS mode operation and analytical separation under isocratic conditions with a total separation time of less than two minutes. The absence of ion suppression was confirmed for both the m/z of ropivacaine and the m/z of the internal standard. The solid phase extraction protocol was optimised for elution of ropivacaine in a small volume of aqueous-rich solvent suitable for injection into a reversed phase LC–MS system. The final method measured trace levels of ropivacaine in human plasma with a limit of quantification of 2.5 nmol/L and interassay accuracy and precision of 101.7–104.4% and 2.1–7.2%, respectively.

Keywords

Molecular imprinting Solid phase extraction Mass spectrometry Bioanalysis Affinity separation Ion suppression 

Notes

Acknowledgements

Z.C. acknowledges the financial support provided through the European Community's Human Potential Programme under contract HPRM-CT-2002-00189 [AquaMIP]. We also thank Dr. Sellergren and his research group for the preparation of the molecularly imprinted polymers.

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Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.BioscienceAstraZeneca R&D SödertäljeSödertäljeSweden
  2. 2.Bioanalytical ServicesCovance Laboratories LtdHarrogateUK

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