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Glucose oxidase–magnetite nanoparticle bioconjugate for glucose sensing

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Abstract

Immobilization of bioactive molecules on the surface of magnetic nanoparticles is of great interest, because the magnetic properties of these bioconjugates promise to greatly improve the delivery and recovery of biomolecules in biomedical applications. Here we present the preparation and functionalization of magnetite (Fe3O4) nanoparticles 20 nm in diameter and the successful covalent conjugation of the enzyme glucose oxidase to the amino-modified nanoparticle surface. Functionalization of the magnetic nanoparticle surface with amino groups greatly increased the amount and activity of the immobilized enzyme compared with immobilization procedures involving physical adsorption. The enzymatic activity of the glucose oxidase-coated magnetic nanoparticles was investigated by monitoring oxygen consumption during the enzymatic oxidation of glucose using a ruthenium phenanthroline fluorescent complex for oxygen sensing. The glucose oxidase-coated magnetite nanoparticles could function as nanometric glucose sensors in glucose solutions of concentrations up to 20 mmol L−1. Immobilization of glucose oxidase on the nanoparticles also increased the stability of the enzyme. When stored at 4°C the nanoparticle suspensions maintained their bioactivity for up to 3 months.

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Acknowledgements

We thank Dr Brian Cushing and Professor Weilie Zhou for their assistance in the X-ray diffraction and TEM measurements. This work was supported by DARPA Grant no. MDA -972-03-C-0100 and by NSF Grant no. CHE-0314027

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Authors and Affiliations

  1. Department of Chemistry and the Advanced Material Research Institute (AMRI), University of New Orleans, New Orleans, LA, 70148, USA

    Liane M. Rossi, Ashley D. Quach & Zeev Rosenzweig

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  1. Liane M. Rossi
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  2. Ashley D. Quach
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  3. Zeev Rosenzweig
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Correspondence to Zeev Rosenzweig.

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Rossi, L.M., Quach, A.D. & Rosenzweig, Z. Glucose oxidase–magnetite nanoparticle bioconjugate for glucose sensing. Anal Bioanal Chem 380, 606–613 (2004). https://doi.org/10.1007/s00216-004-2770-3

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  • DOI: https://doi.org/10.1007/s00216-004-2770-3

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