Continuous solid-phase extraction and preconcentration of bisphenol A in aqueous samples using molecularly imprinted columns
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A bisphenol A (BPA) molecularly imprinted polymer, the composition of which was optimised using a chemometric approach, has been applied to the selective preconcentration of the template from aqueous samples. The selectivity of the polymer toward BPA and related compounds was evaluated chromatographically. The BPA-imprinted polymer was packed in a column and used for continuous on-column solid-phase extraction (MISPE) of aqueous samples followed by subsequent analysis by HPLC with fluorescence detection of the eluted fractions. The composition of the washing solvent applied in the MISPE procedure was optimised to favour the specific interactions of the MIP with BPA and to remove the non-selectively bound matrix components. The MISPE method has proven to be effective for selective preconcentration of BPA in aqueous samples (recoveries >84% obtained in the eluate for 10–100 mL sample volumes) enabling detection and quantification limits of 1.0 and 3.3 ng mL−1, respectively (based on 25 mL sample size). Analytical recoveries were between 92 and 101% for river water samples spiked with known amounts of BPA (30, 60, and 80 ng mL−1); relative standard deviations (RSD) were lower than 5.0%.
KeywordsBisphenol A Molecularly imprinted polymer MISPE Water analysis Endocrine disruptors
The authors gratefully acknowledge the Madrid Community (ref. CAM-07 M/0082/00) and the Spanish Ministry of Science and Technology (Project No. BQU2002-04515-C02-01) for financial support. B.San Vicarte and F.Navarro Villoshada thank Madrid Community for a post-doctoral grant.
- 1.Dermer OC, McKelta JJ, Cunningham WA (eds) (1977) Encyclopaedia of chemical processing and design, vol 4. Marcel Dekker, New YorkGoogle Scholar
- 3.Staples CA, Woodburn K, Caspers N, Hall AT, Dorn PB, Klecka GM (2002) Hum Ecol Risk Assess 8:1083–1105Google Scholar
- 4.European Union (2001) Risk assessment of 2,2-bis(4-hydroxyphenyl)propane (bisphenol A): human health effects. Document prepared by the UK health and safety executive on behalf of the European union under the existing substances regulation (council regulation EEC/793/93). Draft reportGoogle Scholar
- 17.Lanza F, Sellergren B (2001) Chromatographia 53:599–611Google Scholar
- 18.Sellergren B (ed) (2001) Molecularly imprinted polymers: man-made mimics of antibodies and their application in analytical chemistry. Elsevier, AmsterdamGoogle Scholar