Analytical and Bioanalytical Chemistry

, Volume 380, Issue 2, pp 293–302 | Cite as

Influence of cetyltrimethylammonium bromide on phosphatidylcholine-coated capillaries

  • Ruth Kuldvee
  • Maria V. Lindén
  • Susanne K. Wiedmer
  • Marja-Liisa Riekkola
Original Paper
First Online:
Received:
Revised:
Accepted:

Abstract

Large unilamellar vesicles of egg-phosphatidylcholine (eggPC), a naturally occurring phospholipid, were used in capillary electrophoresis (CE) for semi-permanent coating of fused silica capillaries. The stability of the phospholipid coating was tested at different cetyltrimethylammonium bromide (CTAB) concentrations with and without CaCl2 present in the coating solution. The effect of physical factors influencing the coating stability (e.g. duration of the coating time, storage temperature of the coating solution) were also studied. Standing overnight in background electrolyte (BGE) solution did not alter the eggPC phospholipid coating noticeably. The performance of the coating was tested with a mixture of basic proteins (lysozyme, ribonuclease A and α-chymotrypsinogen A). Highest efficiencies (over 200,000 plates m−1) were achieved when the capillary was filled for 15 h with a liposome solution containing both CTAB and CaCl2.

Keywords

Capillary electrophoresis Liposomes Phospholipids Semi-permanent coating CTAB Protein separation 
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Notes

Acknowledgements

Financial support was provided by the Academy of Finland under grants SA 73357 (MLR), 202216 (SKW, RK, MVL) and 78785 (SKW).

Supplementary material

216_2004_2722.pdf

216_2004_2722.pdf
(pdf 69 KB)

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ruth Kuldvee
    • 1
  • Maria V. Lindén
    • 1
  • Susanne K. Wiedmer
    • 1
  • Marja-Liisa Riekkola
    • 1
  1. 1.Laboratory of Analytical Chemistry, Department of ChemistryUniversity of HelsinkiHelsinki 00014Finland

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