Application of SEC-ICP-MS for comparative analyses of metal-containing species in cancerous and healthy human thyroid samples
- 287 Downloads
Size exclusion chromatography (SEC) was coupled on-line to inductively coupled plasma mass spectrometry (ICP-MS) for speciation study of trace metals in cancerous thyroid tissues in comparison to healthy thyroids aimed to estimation of changes in metalloprotein speciation in pathological tissue. The study showed a presence of species binding Cu, Zn, Cd and Pb in healthy thyroid tissue with a good reproducibility of chromatographic results, whereas the same species could not be detected in cancerous tissues. Thus, remarkable differences with respect to metal-binding species were revealed between healthy and pathological thyroid samples, pointing out a completely different distribution of trace metals in cancerous tissues. The metal-binding species could not be identified in the frame of this work because of a lack of appropriate standards. Nevertheless, the results obtained confirm the suitability of SEC-ICP-MS for monitoring of changes in trace metal distribution in cancerous tissue and will help to better understand the role of metal-containing species in thyroid pathology.
KeywordsThyroid cancer Metal speciation Metalloprotein Size exclusion chromatography Inductively coupled plasma mass spectrometry
This work was carried out within the DFG-Graduiertenkolleg 826 Trace analysis of elemental species: method developments and applications. The authors therefore thank the German Research Council (DFG) for financial support. The authors are grateful to Prof. A.F. Malenchenko for preparation of thyroid samples.
- 5.Hellquist HB (1997) Acta Otolaryngol 527:25–29Google Scholar
- 8.Karaoglou A, Desmet G, Kelly GN, Menzel HG (1996) (eds) The Radiological Consequences of the Chernobyl Accident. Part IIIB: thyroid cancer in children living near chernobyl. EUR 16544, Luxembourg, pp 669–834Google Scholar
- 9.Williams ED, Tronko ND (eds) (1996) Molecular, cellular, biological characterisation of childhood thyroid cancer. EUR 16538, LuxembourgGoogle Scholar
- 10.UNSCEAR (2000) Exposures and effects of the Chernobyl accident, in sources and effects of ionizing radiation. United Nation Publication E.00.IX.4, pp 451–565Google Scholar
- 11.Malko MV (2002) Report KUR-KR-79. Kyoto University, Japan, pp 240–255Google Scholar
- 12.Boulyga SF, Petri H, Zhuk IV, Kanash NV, Malenchenko AF (1999) J Radioanal Nucl Chem 242:335–340Google Scholar
- 13.Boulyga SF, Becker JS, Malenchenko AF, Dietze HJ (2000) Mikrochim Acta 134:215–222Google Scholar
- 14.Cornelis R, Caruso J, Crews H, Heumann KG (eds) (2003) Handbook of element speciation. Wiley, ChichesterGoogle Scholar
- 15.Bettmer J, Brüchert W (2002) Nachr Chem 50:846–848Google Scholar
- 20.Malenchenko AF (ed) (1999) Ecological problems of pediatrics in a large industrial city. Asveta, Minsk (in Russian)Google Scholar