Analytical and Bioanalytical Chemistry

, Volume 379, Issue 7–8, pp 1106–1112 | Cite as

Ultrasound-assisted extraction for the analysis of phenolic compounds in strawberries

  • M. C. Herrera
  • M. D. Luque de CastroEmail author
Original Paper


A semiautomatic method based on application of ultrasounds has been developed to leach and hydrolyse phenolic compounds, such as naringin, rutin, naringenin, ellagic acid, quercetin and kaempferol, from strawberries. Two grams of lyophilized sample was placed into a sample cell and 5 mL of acetone containing hydrochloric acid was added. The cell was immersed in a water bath and sonicated for 30 s (duty cycle 0.8 s, output amplitude 50% of the nominal amplitude of the converter, applied power 100 W and with the probe placed 2 cm from the top surface of the extraction cell) for three times: each time 5 mL extractant displaced the previous extract. When the extraction was completed, the combined extracts were evaporated for 10 min, diluted to 10 mL with water adjusted to pH 8, and transferred to a cleanup–preconcentration manifold; here the analytes were retained in two in-series minicolumns packed with HR-P sorbent and then eluted with 4 mL methanol, and injected for individual separation–quantitation into a chromatograph–photodiode array detector assembly. Optimisation of the extraction was carried out using samples spiked with 4 mg kg−1 of each analyte. Calibration curves using the standard addition in red strawberries typically gave linear dynamic ranges of 4–40 mg L−1 for all analytes, except for ellagic acid (40–400 mg L−1). The r2 values exceeded 0.98 in all cases.


Ultrasound-assisted extraction Flavonoids Flavonols 



Spain’s Comisión Interministerial de Ciencia y Tecnología (CICyT) is gratefully acknowledged for financial support (project BQU-2002-01333).


  1. 1.
    Bjeldanes LF, Chang GW (1997) Mutagenic activity of quercetin and related compounds. Science, Washinton, DC 197:577Google Scholar
  2. 2.
    Stavric B (1984) Mutagenic food flavonoids. Fed Proc 43:2454PubMedGoogle Scholar
  3. 3.
    Van der Hoeven JCM, Bruggeman IM, Debets FMH (1984) Genotoxicity of quercetin in cultured mammalian cells. Mutat Res 136:9PubMedGoogle Scholar
  4. 4.
    MacGregor JT (1984) Genetic and carcinogenic affects of plant flavonoids: an overview. Adv Exp Med Biol 177:497PubMedGoogle Scholar
  5. 5.
    Huang M-T, Wood AW, Newmark HL, Sayer JM, Yagi H, Jerina DM, Conney AH (1983) Inhibition of the mutagenicity of bay-region diol-epoxides of polycyclic aromatic hydrocarbons by phenolic plant flavonoids. Carcinogenesis 4:1631PubMedGoogle Scholar
  6. 6.
    Francis AR, Shetty TK, Bhattacharga RK (1989) Modifying role of dietary factors on the mutagenicity of aflatoxin B1: in vivo effect of plants flavonoids. Mutat Res 222:393CrossRefPubMedGoogle Scholar
  7. 7.
    Deschner EE , Ruperto J, Wong G, Newmark HL (1991) Quercetin and rutin as inhibitors of azoxymethanol-induced colonic neoplasia. Carcinogenesis 7:1193Google Scholar
  8. 8.
    Strack D (1997) Phenolic metabolism In: Dey PM, Harborne JB (eds) Plant biochemistry. Academic, London, p 387Google Scholar
  9. 9.
    Bennet RC, Wallsgrove RM (1994) Secondary metabolites in plant defence mechanisms. Tansley review, 72 New Phytol 127:617Google Scholar
  10. 10.
    Dixon RA, Paiva NL (1995) Stress-induced phenylpropanoid metabolism. Plant Cell 7:1085Google Scholar
  11. 11.
    Bomser J, Madhavi DL, Singletary K, Smith MAL (1996) Planta Med 62:212PubMedGoogle Scholar
  12. 12.
    Häkkinen S, Auriola S (1998) J Chromatogr A 829:91CrossRefPubMedGoogle Scholar
  13. 13.
    Häkkinen S, Törrönen AK (2000) Food Res Int 33:517CrossRefGoogle Scholar
  14. 14.
    Dugo P, Modello L, Errante G, Zappia G, Dugo G (2000) J Agric Food Chem 49:3987CrossRefGoogle Scholar
  15. 15.
    Revilla E, Ryan JM (2000) J Chromatogr A 881:461Google Scholar
  16. 16.
    Estribano-Bailón T, Gutiérrez-Fernández Y, Rivas-Gonzalo J, Santos-Buelga C (1992) J Agric Food Chem 40:1794Google Scholar
  17. 17.
    Antolovich M, Prensler P, Robards K, Ryan D (2000) Analyst 125:989CrossRefGoogle Scholar
  18. 18.
    Häkkinen S, Karenlampi S, Heinoner I, Mykkänen H, Törrönen A (1998) J Sci Food Agric 77:543CrossRefGoogle Scholar
  19. 19.
    Palma M, Piñeiro Z, Barroso CG (2002) J Chromatogr A 968:1CrossRefPubMedGoogle Scholar
  20. 20.
    Llompert MP, Lorenzo RA, Cela R, Li K, Bálanger JMR, Paré JRJ (1997) J Chromatogr A 744:243CrossRefGoogle Scholar
  21. 21.
    Mierzura J, Sun YC, Yang MH (1997) Anal Chim Acta 355:277CrossRefGoogle Scholar
  22. 22.
    Ashley K, Andrews RN, Cavazos L, Demenge M (2001) J Anal At Spectrom 16:1147CrossRefGoogle Scholar
  23. 23.
    Vinodgopal K, Peller J, Makogon O, Hieffje GM (1998) Appl Spectrosc 52:515Google Scholar
  24. 24.
    Luque de Castro MD, Luque-García JL (2002) Acceleration and automation of solid sample treatment, Elsevier, AmsterdamGoogle Scholar
  25. 25.
    Commision Directive of 24 July 1979 establishing community methods of sampling of the official control of pesticide residues in and on fruit and vegetables 79/700/EEC. Official Journal of European Community, European Community, BrusselsGoogle Scholar
  26. 26.
    Hakkinen SH, Karenlampi SO, Heinonen IM, Mykkanen HM, Torronen AR (1999) J Agric Food Chem 47:2274PubMedGoogle Scholar
  27. 27.
    Justense U, Knuthsen P, Leth T (1998) J Chromatogr A 799:101CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.Analytical Chemistry Department, Annex C-3, Campus of RabanalesUniversity of CórdobaCórdobaSpain

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