Analytical and Bioanalytical Chemistry

, Volume 379, Issue 2, pp 255–260 | Cite as

Application of heated electrodes operating in a non-isothermal mode for interference elimination with amperometric biosensors

  • Carolin Lau
  • Sabine Reiter
  • Wolfgang Schuhmann
  • Peter Gründler
Special Issue Paper


Heated electrodes were applied for the non-isothermal operation of amperometric glucose biosensors based on glucose oxidase immobilised on the electrode surface by entrapment within a polymer layer. The localised deposition of the polymer film under simultaneous entrapment of the enzyme was achieved by an electrochemically induced pH-modulation in the diffusion zone in front of the electrode, thus altering the solubility of the polymer chains. This non-manual sensor preparation protocol could be successfully used for the modification of a novel indirectly heated electrode. The non-isothermal operating mode allows working at the optimum temperature of the enzyme sensors without any thermal distortion of the bulk solution. Increased surface temperature of the sensor thus accelerates transport as well as kinetic processes, resulting in an enhanced amperometric signal.

In the presence of interfering compounds such as ascorbic acid, the proposed technique allows use of the diverging thermal impact on the sensing process, for different electrochemically active compounds, for a deconvolution of the amperometric signal at different electrode temperatures. A calculation method for determination of glucose in the presence of one interfering compound is presented as a basis for a calculative interference elimination.


Heated electrodes Glucose biosensor Interference Ascorbic acid 



The authors thank TETEKERA company for providing the LTCC electrodes and Mrs. S. Janiak for technical assistance. Financial support of Deutsche Forschungsgemeinschaft (Project Gr1238/6–4) as well as of “Allgemeines Promotionskolleg der Ruhr-Universität Bochum” is gratefully acknowledged.


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Carolin Lau
    • 1
  • Sabine Reiter
    • 2
  • Wolfgang Schuhmann
    • 2
  • Peter Gründler
    • 1
  1. 1.Fachbereich Chemie, Abteilung für Analytische, Technische und UmweltchemieUniversität RostockRostockGermany
  2. 2.Anal. Chem.—Elektroanalytik & SensorikRuhr-Universität BochumBochumGermany

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