Analytical and Bioanalytical Chemistry

, Volume 378, Issue 7, pp 1678–1692 | Cite as

Micro- and nanofluidics for DNA analysis

  • Jonas O. TegenfeldtEmail author
  • Christelle Prinz
  • Han Cao
  • Richard L. Huang
  • Robert H. Austin
  • Stephen Y. Chou
  • Edward C. Cox
  • James C. Sturm
Paper in Forefront


Miniaturization to the micrometer and nanometer scale opens up the possibility to probe biology on a length scale where fundamental biological processes take place, such as the epigenetic and genetic control of single cells. To study single cells the necessary devices need to be integrated on a single chip; and, to access the relevant length scales, the devices need to be designed with feature sizes of a few nanometers up to several micrometers. We will give a few examples from the literature and from our own research in the field of miniaturized chip-based devices for DNA analysis, including dielectrophoresis for purification of DNA, artificial gel structures for rapid DNA separation, and nanofluidic channels for direct visualization of single DNA molecules.


PDMS Microfluidic Channel Electron Beam Lithography Relevant Length Scale Entropic Barrier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are indebted to Zhaoning Yu for making high-quality nanostructured surfaces using nanoimprinting lithography. The authors are especially indebted to the following colleagues for fruitful discussions. Olgica Bakajin, Lawrence Livermore National Laboratories, CA; Shirley S. Chan, Princeton, NJ; Prof Chia-Fu Chou, Arizona State University, Tempe, AZ; Prof H. C. Craighead at Cornell, Ithaca, NY; Nicholas C. Darnton at the Rowland Institute at Harvard, Cambridge, MA; Thomas A.J. Duke at Cavendish Laboratory, Cambridge, UK; J.J. Kraeft, Princeton University, NJ; Robert Riehn, Princeton University, NJ; Walter W. Reisner, Princeton University, NJ; Pascal Silberzan at the Institut Curie, Paris, France; and Yan Mei Wang, Princeton University, NJ.

The work was funded by grants from the Defense Advanced Research Projects Agency (MDA972–00–1-0031), the National Institutes of Health (HG01506), the state of New Jersey (NJCST 99–100–082–2042–007), and the Nanobiotechnology Center (NSF BSCECS9876771).


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Jonas O. Tegenfeldt
    • 1
    • 4
    Email author
  • Christelle Prinz
    • 2
    • 5
  • Han Cao
    • 3
    • 6
  • Richard L. Huang
    • 3
  • Robert H. Austin
    • 2
  • Stephen Y. Chou
    • 3
  • Edward C. Cox
    • 1
  • James C. Sturm
    • 3
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA
  2. 2.Department of PhysicsPrinceton UniversityPrincetonUSA
  3. 3.Department of Electrical EngineeringPrinceton UniversityPrincetonUSA
  4. 4.The Division of Solid State PhysicsLund UniversityLundSweden
  5. 5.The Division of Solid State PhysicsLund UniversityLundSweden
  6. 6.BioNanomatrixBlawenburgUSA

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