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Analytical and Bioanalytical Chemistry

, Volume 378, Issue 8, pp 1929–1934 | Cite as

Bioimprinted QCM sensors for virus detection—screening of plant sap

  • Franz L. DickertEmail author
  • Oliver Hayden
  • Roland Bindeus
  • Karl-J. Mann
  • Dieter Blaas
  • Elisabeth Waigmann
Original Paper

Abstract

Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL−1 to 1 mg mL−1 within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.

Keywords

Tobacco mosaic virus Quartz-crystal microbalance Molecular imprinting Atomic force microscopy (AFM) 

Notes

Acknowledgement

This work was supported by FWF (P 15512).

References

  1. 1.
    Thompson JR, Wetzel S, Klerks MM, Vasková D, Schoen CD, Spak J, Jelkmann W (2003) J Virol Methods 11:85–93CrossRefGoogle Scholar
  2. 2.
    Uzagara SG (2000) Biotechnol Adv 18:179–206CrossRefPubMedGoogle Scholar
  3. 3.
    Nutter Jr FW (1997) Crop Protect 16:603–618CrossRefGoogle Scholar
  4. 4.
    Dickert FL, Tortschanoff M, Bulst WE, Fischerauer G (1999) Anal Chem 71:4559–4563CrossRefGoogle Scholar
  5. 5.
    Pereda S, Ehrenfeld N, Median C, Delgado J, Arce-Johnson P (2000) J Virol Methods 90:135–142CrossRefPubMedGoogle Scholar
  6. 6.
    Fabre F, Kervarrec C, Mieuzet L, Riault G, Vialatte A, Emmanuel J (2003) J Virol Methods 110:51–60CrossRefPubMedGoogle Scholar
  7. 7.
    Eun AJC, Huang L, Chew FT, Li SFY, Wong SM (2002) J Virol Methods 99:71–79CrossRefPubMedGoogle Scholar
  8. 8.
    Jin-Cherng Eun A, Huang L, Chew FT, Fong-Yau Li S, Sek-Man W (2003) J Virol Methods 99:71–79Google Scholar
  9. 9.
    Liu YC, Wang CM, Hsiung KP, Chienjin H (2003) Biosens Bioelectron 18:937–942CrossRefPubMedGoogle Scholar
  10. 10.
    Drygin YF, Korotaeva SG, Dorokhov YL (1992) FEBS 309:350–352CrossRefGoogle Scholar
  11. 11.
    Dickert FL, Hayden O, Halikias K (2001) Analyst 126:766–771CrossRefPubMedGoogle Scholar
  12. 12.
    Hayden O, Dickert FL (2001) Adv Mater 13:1480–1483CrossRefGoogle Scholar
  13. 13.
    Dickert FL, Hayden O (2002) Anal Chem 74:1302–1306CrossRefPubMedGoogle Scholar
  14. 14.
    Wulff G (1995) Angew Chem Int Ed Engl 34:1812–1832Google Scholar
  15. 15.
    Kempe M, Glad M, Mosbach K (1995) J Mol Recognit (1995) 8:35–39Google Scholar
  16. 16.
    Aherne A, Alexander C, Payne MJ, Perez N, Vulfson EN (1996) J Am Chem Soc 118:8771–8772CrossRefGoogle Scholar
  17. 17.
    Fraenkel-Conrat H, Williams RC (1955) Proc Natl Acad Sci USA 41:690–698Google Scholar
  18. 18.
    Dickert FL, Halikias K, Hayden O, Liu P, Sikorski R (2001) Sens Actuators B 76:295–298CrossRefGoogle Scholar
  19. 19.
    Martin SJ, Frye GC, Ricco AJ, Senturia SD (1993) Anal Chem 65:2910–2922Google Scholar
  20. 20.
    Sakti SP, Rösler S, Lucklum R, Hauptmann P, Bühling F, Ansorge S (1999) Sens Actuators A 76:98–102CrossRefGoogle Scholar
  21. 21.
    Hayden O, Bindeus R, Dickert FL (2003) Meas Sci Technol 14:1876–1881CrossRefGoogle Scholar
  22. 22.
    Lucklum R, Behling C, Hauptmann P (1999) Anal Chem 71:2488–2496CrossRefGoogle Scholar
  23. 23.
    Sakti SP, Lucklum R, Hauptmann P, Bühling F, Ansorge S (2001) Biosens Bioelectron 16:1101–1108CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Franz L. Dickert
    • 1
    Email author
  • Oliver Hayden
    • 1
  • Roland Bindeus
    • 1
  • Karl-J. Mann
    • 1
  • Dieter Blaas
    • 2
  • Elisabeth Waigmann
    • 2
  1. 1.Institute of Analytical ChemistryVienna UniversityViennaAustria
  2. 2.Institute of Medical BiochemistryVienna UniversityWienAustria

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