Analytical and Bioanalytical Chemistry

, Volume 378, Issue 8, pp 1929–1934 | Cite as

Bioimprinted QCM sensors for virus detection—screening of plant sap

  • Franz L. DickertEmail author
  • Oliver Hayden
  • Roland Bindeus
  • Karl-J. Mann
  • Dieter Blaas
  • Elisabeth Waigmann
Original Paper


Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL−1 to 1 mg mL−1 within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.


Tobacco mosaic virus Quartz-crystal microbalance Molecular imprinting Atomic force microscopy (AFM) 



This work was supported by FWF (P 15512).


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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Franz L. Dickert
    • 1
    Email author
  • Oliver Hayden
    • 1
  • Roland Bindeus
    • 1
  • Karl-J. Mann
    • 1
  • Dieter Blaas
    • 2
  • Elisabeth Waigmann
    • 2
  1. 1.Institute of Analytical ChemistryVienna UniversityViennaAustria
  2. 2.Institute of Medical BiochemistryVienna UniversityWienAustria

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