Bioimprinted QCM sensors for virus detection—screening of plant sap
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Surface imprinting techniques on polymer-coated quartz-crystal microbalances (QCM) have been used to detect tobacco mosaic viruses (TMV) in aqueous media. Molecularly imprinted polymers (MIP), tailor-made by self organisation of monomers around a template (TMV), were generated directly on the gold electrodes. Imprinted trenches on the polymer surface mimicking the shape and surface functionality of the virus serve as recognition sites for re-adsorption after washing out of the template. The sensors are applicable to TMV detection ranging from 100 ng mL−1 to 1 mg mL−1 within minutes. Furthermore, direct measurements without time-consuming sample preparation are possible in complex matrices such as tobacco plant sap.
KeywordsTobacco mosaic virus Quartz-crystal microbalance Molecular imprinting Atomic force microscopy (AFM)
This work was supported by FWF (P 15512).
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