On-line LC-GC and comprehensive two-dimensional LCxGC-ToF MS for the analysis of complex samples
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Liquid chromatography (LC) and gas chromatography (GC) are by far the most frequently applied analytical methods in (organic) chemical analysis. To some extent LC and GC are competing techniques. With the exception of permanent gases, all analytes that can be separated using GC can also be subjected to LC. On the other hand, with the exception of high molecular-weight, highly polar or thermally labile analytes, many LC applications can also be handled by GC. Rather than considering the two chromatographic methods as competitors, hyphenated LC-GC exploits the complementary nature of LC and GC [1, 2]. LC has a high sample capacity and provides a wide range of separation mechanisms that can be utilized for selective isolation of (groups of) compounds. GC, on the other hand, offers a high separation efficiency as well as a wide variety of selective and sensitive detection devices. In LC-GC the compounds of interest are isolated from the complex matrix and are transferred...