Analytical and Bioanalytical Chemistry

, Volume 378, Issue 8, pp 1913–1921 | Cite as

Peptide recognition via hierarchical imprinting

  • Maria Magdalena Titirici
  • Börje Sellergren
Original Paper


Silica particles containing immobilised peptidic templates have been used for the generation of hierarchically imprinted polymers. The pores of the silica mould were filled with a mixture of monomers/initiator and polymerised, followed by dissolution of the silica template. This method leaves behind imprinted polymers with binding sites located at the surface, which are capable of recognising larger molecules with the same immobilised epitope. All the products resulting from solid-phase synthesis of peptides were characterised by elemental analysis, FT-IR spectroscopy and fluorescence microscopy. The hierarchically imprinted polymers generated from these products were characterised by elemental analysis, FT-IR spectroscopy, fluorescence microscopy, scanning electron microscopy (SEM) and nitrogen adsorption, providing evidence concerning the reproducibility of each step. The chromatographic properties of the materials have been investigated and the advantages of the immobilisation method have been proven. The materials exhibit selectivity for their templates and other structurally related dipeptides. Furthermore, the polymers proved to be capable of recognising larger peptides containing the immobilised sequence.


Molecularly imprinted polymer Hierarchical imprinting Peptide detection Solid-phase synthesis Liquid chromatography 



We thank Gunnar Glasser (MPI für Polymerforschung, Mainz) for recording of the SEM micrographs. Financial contribution from the Deutsche Forschungsgemeinschaft (DFG) under Project 777/5–1 is gratefully acknowledged.


  1. 1.
    Sellergren B (ed) (2001) Molecularly imprinted polymers. Man made mimics of antibodies and their applications in analytical chemistry. Elsevier, Amsterdam, vol 23; Wulff G (2002) Chem Rev 102(1):1CrossRefPubMedGoogle Scholar
  2. 2.
    Perez N, Whitcombe MJ, Vulfson EN (2001) Macromolecules 34:30CrossRefGoogle Scholar
  3. 3.
    Matsui J, Okada M, Tsuruoka M, Takeutchi T (1997) Anal Commun 34:85CrossRefGoogle Scholar
  4. 4.
    Ye L, Cormack P, Mosbach K (1999) Anal Com 36:35; Tamayo FG, Casillas JL, Martin- Esteban A (2003) Anal Chim Acta 482(2):165Google Scholar
  5. 5.
    Sulitzky C, Rücket B, Hall AJ, Lanza F, Unger KK, Sellergren B (2002) Macromolecules 35(1):79; Sellergren B, Rückert B, Hall AJ (2002) Adv Mat14:1204Google Scholar
  6. 6.
    Dai S, Burleight MC, Ju YH, Gao HJ, Lin JS, Pennycook SJ, Barnes CE, Xue ZL (2000) J Am Chem Soc 122:992CrossRefGoogle Scholar
  7. 7.
    Yilmaz E, Haupt K, Mosbach K (2000) Angew Chem Int Ed 39(12):2115CrossRefPubMedGoogle Scholar
  8. 8.
    Sellergren B, Büchel G (1999) WO01/32760 A1Google Scholar
  9. 9.
    Titirici MM, Hall AJ, Sellergren B (2002) Chem Mater 14:21CrossRefGoogle Scholar
  10. 10.
    Venton DL, Gudipati E (1996) Biochim Biophys Res Commun 227:419CrossRefGoogle Scholar
  11. 11.
    Hjerten S, Liao J-L, Nakazato K, Wang Y, Zamaratskaia G, Zhang H-Y (1997) Chromatographia 44:227Google Scholar
  12. 12.
    Hirayama K, Burow M, Morikawa Y, Minoura N (1998) Chem Lett 731Google Scholar
  13. 13.
    Titirici MM, Hall AJ, Sellergren B (2003) Chem Mater 15:822CrossRefGoogle Scholar
  14. 14.
    Carlier E, Guyot A, Revillon A (1991) React Polym 16:115CrossRefGoogle Scholar
  15. 15.
    Vansant EF, Van Der Voort P, Vrancken KC (1995) Characterisation and chemical modification of the silica surface. Elsevier, Amsterdam, vol 93Google Scholar
  16. 16.
    Sherrington DC, Hodge P (1998) Synthesis and separation using functional polymers. Wiley, ChichesterGoogle Scholar
  17. 17.
    Kaiser E, Picart F, Kubiak T, Tam JP, Merrifield RB (1993) J Org Chem 58:5167Google Scholar
  18. 18.
    Sellergren B, Shea KJ (1993) J Chromatogr 635:31CrossRefGoogle Scholar
  19. 19.
    Gagne MR, Becker JJ, Brunkan NM (2000) Polym Prep 41:404Google Scholar
  20. 20.
    Rachckov A, Minoura N (2001) Biochem Biophys Acta 1544:255PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  1. 1.INFUUniversität DortmundDortmundGermany

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