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Analytical and Bioanalytical Chemistry

, Volume 378, Issue 1, pp 104–118 | Cite as

Current strategies for electrochemical detection of DNA with solid electrodes

  • Patricia de-los-Santos-Álvarez
  • M. Jesús Lobo-Castañón
  • Arturo J. Miranda-Ordieres
  • Paulino Tuñón-BlancoEmail author
Review

Abstract

A review of current strategies aimed at detecting nucleic acids (NA) using NA-modified solid electrodes reveals the versatility and potential of electrochemical detection in this field. What emerged at the beginning of 90s as a very promising detection system in DNA technology is now resulting in the first commercial devices. Many aspects of the experimental design, for example surface immobilisation and detection schemes, are outlined and evaluated. Although most approaches use hybridisation as the recognition reaction, those not based on hybridisation are also included. As is finally shown, great advances have been achieved, although further developments are required if electrochemical devices are to be suitable for routine measurement.

Keywords

Electrochemical detection of NA DNA-modified electrodes Electrochemical mutation detection 

Abbreviations

A

Adenine

a.c.V

Alternating current voltammetry

Amp.

Amperometry

Apo E

Apolipoprotein E

BCB

Brilliant cresyl blue

BLM

Bilayer lipid membrane

bp

Base pairs

BPPG

Basal planar pyrolytic graphite

bpy

2,2′-Bipyridine

C

Cytosine

CMV

Citomegalovirus

CPE

Carbon paste electrode

CPSA

Constant-current chronopotentiometric stripping analysis

Cronoamp.

Chronoamperometry

CSPE

Carbon screen-printed electrode

CV

Cyclic voltammetry

dmb

4,4′-Dimethyl-2,2′-bipyridine

dmphen

Dimethylphenanthroline

DPPZ

Dipyrido[3,2-a.2′,3′-c]phenazine

DPV

Differential pulse voltammetry

dsDNA

Double-stranded DNA

EtBr

Ethidium bromide

Fc

Ferrocene

Fc-CA

Ferrocenecarboxaldehyde

Fc-CTPPZ

Di[1-ferrocenecarbamoylpropyl)tetrahydropyrazine-4-(propylcarbamoylpyridine)]phenazine

Fc-NH2

Aminoferrocene

FET

Field-effect transistor

G

Guanine

GCE

Glassy carbon electrode

HBV

Hepatitis B virus

HGH1

Human growth hormone 1

HIV

Human immunodeficiency virus

HRP

Horseradish peroxidase

HOPGE

Highly oriented pyrolytic graphite electrode

IPA

Intermittent pulse amperometry

ISFET

Ion-selective field-effect transistor

ITO

Tin-doped indium oxide electrode

LSV

Linear sweep voltammetry

Nano-Au

Au nanoparticles

NMP

Nucleotide monophosphate

ODN

Oligodeoxynucleotide

ox.

Oxidation

PCR

Polymerase chain reaction

PET

Poly(ethylene terephthalate)

PGE

Pyrolytic graphite electrode

phen

1,10-Phenanthroline

PNA

Peptide nucleic acid

Ppy

Polypyrrole

(PQQ)GDH

Pyrroline quinone glucose dehydrogenase

red.

Reduction

SAM

Self-assembled monolayer

SBP

Soybean peroxidase

SNP

Single nucleotide polymorphism

ssDNA

Single-stranded DNA

SWV

Square-wave voltammetry

T

Thymine

Th

Thionine

TTV

TT virus

ΔEp

Difference of peak potential

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • Patricia de-los-Santos-Álvarez
    • 1
  • M. Jesús Lobo-Castañón
    • 1
  • Arturo J. Miranda-Ordieres
    • 1
  • Paulino Tuñón-Blanco
    • 1
    Email author
  1. 1.Departamento de Química Física y AnalíticaUniversidad de OviedoOviedoSpain

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