Analytical and Bioanalytical Chemistry

, Volume 378, Issue 3, pp 827–833 | Cite as

Determination of two intact glucosinolates in vegetables and Chinese herbs

  • Zongwei Cai
  • Ching-Yan Cheung
  • Wai-Tang Ma
  • Wai-Man Au
  • Xiang You Zhang
  • Albert Lee
Original Paper


A reversed-phase HPLC method was developed for analyzing sinigrin and gluconasturtiin in six vegetable and two Chinese herb samples. A gradient program and mobile phases using methanol and 0.05% trifluoroacetic acid containing 20 mM ammonium acetate allowed sufficient retention and separation of the glucosinolates in the sample extracts. Quadrupole time-of-flight tandem mass spectrometry in negative ion electrospray ionization was used to analyze the fractions collected from the HPLC elution to confirm the identification of the glucosinolates. The levels of sinigrin and gluconasturtiin in the vegetables and Chinese herbs were determined by using an external calibration method. Concentrations of gluconasturtiin in the Chinese herbs were more than 15 times higher than those of sinigrin. Detection limits were 18 nmol g−1 for sinigrin and 4 nmol g−1 for gluconasturtiin when 50 g of fresh vegetable was analyzed.


Glucosinolates HPLC Sinigrin Gluconasturtiin ESI-QTOF-MS 



This research was supported by Faculty Research Grants (FRG/00–01/II-56) and (FRG/01–02/I-19) from Hong Kong Baptist University and the Equipment Grant-Central Allocation of the Research Grants Council, University Grants Committee of Hong Kong (HKBU-1/00C).


  1. 1.
    Fahey JW, Zalcmann AT, Talalay P (2001) Phytochem 56:5CrossRefPubMedGoogle Scholar
  2. 2.
    Rosa EAS, Heaney RK, Fenwick GR, Portas CAM (1997) Horticultural reviews, Vol 19. Wiley, New York, p 99Google Scholar
  3. 3.
    Reichelt M, Brown PD, Schneider B, Oldham NJ, Stauber E, Tokuhisa J, Kliebenstein DJ, Mitchell-Olds T,Gershenzon J (2002) Phytochem 59:663CrossRefGoogle Scholar
  4. 4.
    Kushad MM, Brown AF, Kurilich AC, Juvik JA, Klein BP, Wallig MA, Jeffery EH (1999) J Agric Food Chem 47:1541CrossRefPubMedGoogle Scholar
  5. 5.
    Shen HB, Pang GP, Xie ZP (1987) Zhong Yao Tong Bao 12(4):18Google Scholar
  6. 6.
    Matthaus B, Fiebig HJ (1996) J Agric Food Chem 44:3894CrossRefGoogle Scholar
  7. 7.
    Fenwick GR, Heaney RK, Mullin WJ (1983) Food Sci Nutrition 18:123Google Scholar
  8. 8.
    Zhang Y, Kensler TW, Cho CG, Posner GH, Talaly P (1994) Proc Natl Acad Sci USA 91:3147PubMedGoogle Scholar
  9. 9.
    Bradfield CA, Bjeldanes LF (1987) J Agric Food Chem 35:46Google Scholar
  10. 10.
    Tolra RP, Alonso R, Poschenrieder C, Barcelo D, Barcelo J (2000) J Chromatogr A 889:75CrossRefPubMedGoogle Scholar
  11. 11.
    Karcher A, Melouk HA, Rassi ZE (1999) J Agric Food Chem 47:4267CrossRefPubMedGoogle Scholar
  12. 12.
    Jiao D, Yu MC, Hankin JH, Low SH, Chung FL (1998) J Agric Food Chem 46(3):1055CrossRefGoogle Scholar
  13. 13.
    Chiang WC, Pusateri DJ, Leitz REA (1998) J Agric Food Chem 46(3):1018CrossRefGoogle Scholar
  14. 14.
    Leoni O, Lori R. Palmieri D, Esposito E, Menegatti E, Cortesi R, Nastruzzi C (1997) Bioorg Med Chem 5(9):1799CrossRefPubMedGoogle Scholar
  15. 15.
    Doorn HE, Kruk GC, Holst GJ (1999) J Agric Food Chem 47:1029CrossRefPubMedGoogle Scholar
  16. 16.
    Jezek J, Haggett BGD, Atkinson A, Rawson DM (1999) J Agric Food Chem 47:4669CrossRefPubMedGoogle Scholar
  17. 17.
    Warton B, Matthiessen JN, Shackleton MA (2001) J Agric Food Chem 49(11):5244CrossRefPubMedGoogle Scholar
  18. 18.
    Olivier C, Vaughn SF, Mizubuti ESG, Loria R (1999) J Chem Eco 25(12):2687CrossRefGoogle Scholar
  19. 19.
    Shaw GJ, Andrzejewski D, Roach JAG, Sphon JA (1989) J Agric Food Chem 37:372Google Scholar
  20. 20.
    Underhill EW, Kirkland DF (1971) J Chromatogr 57:47CrossRefGoogle Scholar
  21. 21.
    Minchinton I, Sang J, Burke D, Truscot RJW (1982) J Chromatogr 247:141CrossRefGoogle Scholar
  22. 22.
    Spinks EA, Sones K, Fenwick GR (1984) Fette Seifen Anstrichm 86:228Google Scholar
  23. 23.
    Sang JP, Truscott RJW (1984) J Assoc Off Anal Chem 67:829Google Scholar
  24. 24.
    EC (1990) Off J Eur Commun L170:03.07.27–34Google Scholar
  25. 25.
    Hogge LR, Reed DW, Underhill EW (1988) J Chromatogr Sci 26:348Google Scholar
  26. 26.
    Matthaus B, Luftmann H (2000) J Agric Food Chem 48:2234CrossRefPubMedGoogle Scholar
  27. 27.
    Ishida M, Chiba I, Okuyama Y, Takahata Y, Kaizuma N(1997) JARQ 31:73Google Scholar
  28. 28.
    Lange R, Schumann W, Petrzika M, Busch H, Marquard R (1995) Fat Sci Technol 97:146Google Scholar
  29. 29.
    Prestera T, Fahey JW, Holtzclaw WD, Abeygunawardana C, Kachinski JL, Talalay P (1996) Anal Biochem 2239:168CrossRefGoogle Scholar
  30. 30.
    Arguello LG, Sensharma DK, Qiu F, Nurtaeva A, Rassi ZE (1999) J AOAC Int 82(5):1115Google Scholar
  31. 31.
    Szmigielska AM, Schoenau JJ, Levers V (2000) J Agric Food Chem 48:4487CrossRefPubMedGoogle Scholar
  32. 32.
    Szmigielska AM, Schoenau JJ, Levers V (2000) J Agric Food Chem 48:5190CrossRefPubMedGoogle Scholar
  33. 33.
    Jen JF, Lin TH, Huang JW, Chung WC (2001) J Chromatogr A 912:363CrossRefPubMedGoogle Scholar
  34. 34.
    Rangkadilok N, Nicolas ME, Bennett RN, Premier RR, Eagling DR, Taylor PWJ (2002) Scientia Horticulturae 96:27CrossRefGoogle Scholar
  35. 35.
    Troyer JK, Stephenson KK, Fahey JW (2001) J Chromatogr A 919:299CrossRefPubMedGoogle Scholar
  36. 36.
    Paugam L, Menard R, Larue JP, Thouvenot D (1999) J Chromatogr A 864:155CrossRefPubMedGoogle Scholar
  37. 37.
    Zrybko CL, Fukuda EK, Rosen RT (1997) J Chromatogr 767:43CrossRefGoogle Scholar
  38. 38.
    Skoog DA, Holler FJ, Nieman TA (1998) Principles of instrumental analysis 5th edn. Harcourt Brace, Orlando, p 13Google Scholar

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Zongwei Cai
    • 1
  • Ching-Yan Cheung
    • 1
  • Wai-Tang Ma
    • 1
  • Wai-Man Au
    • 1
  • Xiang You Zhang
    • 1
  • Albert Lee
    • 1
    • 2
  1. 1.Department of ChemistryHong Kong Baptist UniversityKowloonP.R. China
  2. 2.Central Laboratory of the Institute of Molecular Technology for Drug Discovery and SynthesisHong Kong Baptist UniversityKowloonP.R. China

Personalised recommendations